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Literature summary extracted from

  • Saku, T.; Fushinobu, S.; Jun, S.; Ikeda, N.; Nojiri, H.; Yamane, H.; Omori, T.; Wakagi, T.
    Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IP01 (2002), J. Biosci. Bioeng., 93, 568-574 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.9 expressed in Escherichia coli Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.9 S103A the mutant has 100000fold lower activity than that of the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.9 0.0073
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate 150 mM phosphate buffer, at pH 7.5 and 25°C Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O Pseudomonas fluorescens
-
 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O Pseudomonas fluorescens IP01
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.9 Pseudomonas fluorescens P96965
-
-
3.7.1.9 Pseudomonas fluorescens IP01 P96965
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.9
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.9 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O slight hydrolytic activity Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O slight hydrolytic activity Pseudomonas fluorescens IP01 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.9 homodimer 2 * 32000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas fluorescens
3.7.1.9 6-isopropyl-HODA hydrolase
-
 Pseudomonas fluorescens
3.7.1.9 CumD
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.9 21
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate 150 mM phosphate buffer, at pH 7.5 and 25°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.9 8
-
-
 Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.9 7 9 the enzyme activity is stable between pH 7.0 and 9.0 Pseudomonas fluorescens