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Literature summary extracted from

  • Lv, C.; Gu, T.; Xu, K.; Gu, J.; Li, L.; Liu, X.; Zhang, A.; Gao, S.; Li, W.; Zhao, G.
    Biochemical characterization of a beta-N-acetylhexosaminidase from Streptomyces alfalfae and its application in the production of N-acetyl-D-glucosamine (2019), J. Biosci. Bioeng., 128, 135-141 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expressed in Escherichia coli BL21(DE3) cells Streptomyces alfalfae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ 25% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 Ca2+ 92% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 Cu2+ 94% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 EDTA 91% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 Fe2+ 72% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 Hg2+ 5% residual activity at 1 mM Streptomyces alfalfae
3.2.1.52 Zn2+ 56% residual activity at 1 mM Streptomyces alfalfae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.52 Co2+ 104% activity at 1 mM Streptomyces alfalfae
3.2.1.52 Fe3+ 129% activity at 1 mM Streptomyces alfalfae
3.2.1.52 Mg2+ 121% activity at 1 mM Streptomyces alfalfae
3.2.1.52 Mn2+ 111% activity at 1 mM Streptomyces alfalfae
3.2.1.52 additional information not influenced by Ni2+, K+, and Na+ Streptomyces alfalfae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Streptomyces alfalfae A0A3G3NI51
-
-
3.2.1.52 Streptomyces alfalfae ACCC40021 A0A3G3NI51
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 Ni2+-NTA agarose column chromatography Streptomyces alfalfae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 215
-
 with N-acetylchitobiose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 234.9
-
 with N-acetylchitotriose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 242.6
-
 with N-acetylchitohexaose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 253.9
-
 with N-acetylchitopentaose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 395.2
-
 with N-acetylchitotetraose as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 491
-
 with 4-nitrophenyl N-acetyl-beta-D-galactosaminide as substrate, at pH 5.5 and 60°C Streptomyces alfalfae
3.2.1.52 1149.7
-
 with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, at pH 5.5 and 60°C Streptomyces alfalfae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O 42.7% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Streptomyces alfalfae 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O 42.7% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Streptomyces alfalfae ACCC40021 4-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O 100% activity Streptomyces alfalfae 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O 100% activity Streptomyces alfalfae ACCC40021 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 crab shell a-chitin + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 crab shell a-chitin + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N-acetylchitobiose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N-acetylchitobiose + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 N-acetylchitohexaose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose + N-acetylchitopentaose
-
 ?
3.2.1.52 N-acetylchitohexaose + H2O
-
 Streptomyces alfalfae ACCC40021 N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose + N-acetylchitopentaose
-
 ?
3.2.1.52 N-acetylchitopentaose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose + N-acetylchitotetraose
-
 ?
3.2.1.52 N-acetylchitotetraose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose + N-acetylchitotriose
-
 ?
3.2.1.52 N-acetylchitotriose + H2O
-
 Streptomyces alfalfae N-acetyl-beta-D-glucosamine + N-acetylchitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 60000, SDS-PAGE Streptomyces alfalfae
3.2.1.52 ? x * 55664, calculated from amino acid sequence Streptomyces alfalfae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 exo-type beta-N-acetylhexosaminidase
-
 Streptomyces alfalfae
3.2.1.52 Hex
-
 Streptomyces alfalfae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 60
-
-
 Streptomyces alfalfae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 60 65 more than 80% of the maximum activity at 60-65°C Streptomyces alfalfae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 45 50 the enzyme is thermo-stable up to 45°C, retaining 85.3% activity even at 50°C Streptomyces alfalfae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.5
-
-
 Streptomyces alfalfae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 5.9 6 more than 80% of the maximum activity at pH 5.0-6.0 Streptomyces alfalfae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 4.5 8.5 the enzyme retains stable at pH 4.5-8.5, with more than 80 % of activity after 1 h-incubation Streptomyces alfalfae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.52 Streptomyces alfalfae calculated from amino acid sequence
-
 7.9