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Literature summary extracted from

  • Sakai, K.; Kimoto, S.; Shinzawa, Y.; Minezawa, M.; Suzuki, K.; Jindou, S.; Kato, M.; Shimizu, M.
    Characterization of pH-tolerant and thermostable GH 134 beta-1,4-mannanase SsGH134 possessing carbohydrate binding module 10 from Streptomyces sp. NRRL B-24484 (2018), J. Biosci. Bioeng., 125, 287-294 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli BL21 CodonPlus(DE3) cells Streptomyces sp. NRRL B-24484

General Stability

EC Number General Stability Organism
3.2.1.78 although deleting carbohydrate binding module 10 increases the catalytic efficiency, its disruption decreases the pH, solvent and detergent stability of the enzyme Streptomyces sp. NRRL B-24484

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 additional information not influenced by Triton X-100 and Tween-20 (10% (v/v) Streptomyces sp. NRRL B-24484
3.2.1.78 SDS the enzyme shows about 45% activity in the presence of 10% (w/v) SDS Streptomyces sp. NRRL B-24484

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.78 Acetone the enzyme retains 100% activity in the presence of 20% (v/v) acetone Streptomyces sp. NRRL B-24484
3.2.1.78 Ethanol the enzyme retains about 85% activity in the presence of 20% (v/v) ethanol Streptomyces sp. NRRL B-24484
3.2.1.78 isopropanol the enzyme retains about 65% activity in the presence of 20% (v/v) isopropanol Streptomyces sp. NRRL B-24484
3.2.1.78 Methanol the enzyme retains about 80% activity in the presence of 20% (v/v) methanol Streptomyces sp. NRRL B-24484

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Streptomyces sp. NRRL B-24484
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Streptomyces sp. NRRL B-24484

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 nickel-nitrilotriacetic acid agarose column chromatography and HiTrap Q column chromatography Streptomyces sp. NRRL B-24484

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 galactomannan + H2O
-
 Streptomyces sp. NRRL B-24484 ?
-
 ?
3.2.1.78 glucomannan + H2O highest activity Streptomyces sp. NRRL B-24484 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 GH 134 beta-1,4-mannanase
-
 Streptomyces sp. NRRL B-24484
3.2.1.78 GH134
-
 Streptomyces sp. NRRL B-24484

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 40
-
-
 Streptomyces sp. NRRL B-24484

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 100
-
 the enzyme shows 30% and 60% of the maximal activity after an incubation at 100°C for 120 min and 80°C for 60 min, respectively Streptomyces sp. NRRL B-24484

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 120
-
 galactomannan at pH 5.0 and 40°C Streptomyces sp. NRRL B-24484
3.2.1.78 340
-
 glucomannan at pH 5.0 and 40°C Streptomyces sp. NRRL B-24484

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 5
-
-
 Streptomyces sp. NRRL B-24484

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 4 6.5 maximally active between pH 4.0 and 6.5 Streptomyces sp. NRRL B-24484

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 3 10 the enzyme retains more than 80% of the maximum activity after 90 min at 30°C within a pH range of 3.0-10.0 Streptomyces sp. NRRL B-24484