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Literature summary extracted from
- Substrate recognition mechanism of Streptomyces phospholipase D and enzymatic measurement of plasmalogen (2015), J. Biosci. Bioeng., 120, 372-379 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | Triton X-100 | in the presence of 0.05-0.5% and 0.1-0.2% (wt/vol) Triton X-100, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and choline plasmalogen are efficiently hydrolyzed, respectively. Hydrolysis of lysophosphatidylcholine and choline lysoplasmalogen does not require Triton X-100, the hydrolytic activity is inhibited by more than 0.05% (wt/vol) Triton X-100 | Streptomyces sp. NA684 |  |
| 3.1.4.4 | Triton X-100 | in the presence of 0.05-0.5% and 0.1-0.2% (wt/vol) Triton X-100, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and choline plasmalogen are efficiently hydrolyzed, respectively. Hydrolysis of lysophosphatidylcholine and choline lysoplasmalogen does not require Triton X-100, the hydrolytic activity is inhibited by more than 0.05% (wt/vol) Triton X-100 | Streptomyces sp. NA684 | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.4.3 | analysis | enzymatic measurement of choline plasmalogen using PLD684 and phospholipase B | Streptomyces sp. NA684 |
| 3.1.4.4 | analysis | enzymatic measurement of choline plasmalogen using PLD684 and phospholipase B | Streptomyces sp. NA684 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.3 | 0.0513 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.3 | 0.271 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 0.0513 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 0.271 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.3 | extracellular | - |
Streptomyces sp. NA684 | - |
- |
| 3.1.4.4 | extracellular | - |
Streptomyces sp. NA684 | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.3 | Streptomyces sp. NA684 | A0A0E4B8Q4 | bifunctional enzyme, displays activities of EC 3.1.4.3 and EC 3.1.4.4 | - |
| 3.1.4.4 | Streptomyces sp. NA684 | A0A0E4B8Q4 | bifunctional enzyme, displays activities of EC 3.1.4.3 and EC 3.1.4.4 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.3 | from culture supernatant | Streptomyces sp. NA684 |
| 3.1.4.4 | from culture supernatant | Streptomyces sp. NA684 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | the enzyme hydrolyzes 98.4% of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine in 1 h | Streptomyces sp. NA684 | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate + choline | - |
? | |
| 3.1.4.3 | choline plasmalogen + H2O | substrate of EC 3.1.4.3 | Streptomyces sp. NA684 | ? | - |
? | |
| 3.1.4.3 | L-alpha-lysophosphatidylcholine + H2O | substrate of EC 3.1.4.3 | Streptomyces sp. NA684 | ? | - |
? | |
| 3.1.4.3 | additional information | the enzyme prefers mixed micelle substrates to liposomal substrates. The rate-limiting steps of hydrolysis of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and emulsified lysophosphatidylcholine are the bulk step and the surface step, respectively | Streptomyces sp. NA684 | ? | - |
? | |
| 3.1.4.4 | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O | the enzyme hydrolyzes 98.4% of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine in 1 h | Streptomyces sp. NA684 | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate + choline | - |
? | |
| 3.1.4.4 | choline plasmalogen + H2O | substrate of EC 3.1.4.3 | Streptomyces sp. NA684 | ? | - |
? | |
| 3.1.4.4 | L-alpha-lysophosphatidylcholine + H2O | substrate of EC 3.1.4.3 | Streptomyces sp. NA684 | ? | - |
? | |
| 3.1.4.4 | additional information | the enzyme prefers mixed micelle substrates to liposomal substrates. The rate-limiting steps of hydrolysis of mixed micelle 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and emulsified lysophosphatidylcholine are the bulk step and the surface step, respectively | Streptomyces sp. NA684 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.3 | ? | x * 54000, SDS-PAGE | Streptomyces sp. NA684 |
| 3.1.4.4 | ? | x * 54000, SDS-PAGE | Streptomyces sp. NA684 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.3 | 243 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.3 | 4000 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 243 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 4000 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.3 | 4740 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.3 | 14800 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 4740 | - |
L-alpha-lysophosphatidylcholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
| 3.1.4.4 | 14800 | - |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | pH 5.0, 37°C | Streptomyces sp. NA684 | |
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