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Literature summary extracted from

  • Schiano-di-Cola, C.; Rojel, N.; Jensen, K.; Kari, J.; Sorensen, T.H.; Borch, K.; Westh, P.
    Systematic deletions in the cellobiohydrolase (CBH) Cel7A from the fungus Trichoderma reesei reveal flexible loops critical for CBH activity (2019), J. Biol. Chem., 294, 1807-1815 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176 expressed in Aspergillus oryzae Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose competitive inhibition Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 0.089
-
 4-nitrophenyl beta-lactoside at pH 5.0 and 25°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + lactose
-
 ?
3.2.1.176 avicel + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.176 azurine-cross-linked hydroxyethylcellulose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.176 bacterial microcrystalline cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.176 regenerated amorphous cellulose + H2O
-
 Trichoderma reesei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 CBH
-
 Trichoderma reesei
3.2.1.176 Cel7A
-
 Trichoderma reesei
3.2.1.176 cellobiohydrolase I
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 66.9
-
 melting temperature Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.176 0.78
-
 4-nitrophenyl beta-lactoside at pH 5.0 and 25°C Trichoderma reesei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.021
-
 cellobiose at pH 5.0 and 25°C Trichoderma reesei