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Literature summary extracted from

  • Domingues, M.N.; Souza, F.H.M.; Vieira, P.S.; de Morais, M.A.B.; Zanphorlin, L.M.; Dos Santos, C.R.; Pirolla, R.A.S.; Honorato, R.V.; de Oliveira, P.S.L.; Gozzo, F.C.; Murakami, M.T.
    Structural basis of exo-beta-mannanase activity in the GH2 family (2018), J. Biol. Chem., 293, 13636-13649 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.100 overexpression in Escherichia coli BL21(DE3) Xanthomonas citri

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.100 hanging-drop vapor-diffusion method at 18°C Xanthomonas citri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.100 4.2
-
beta-mannan pH 5.5, 40°C Xanthomonas citri
3.2.1.100 5.11
-
mannohexaose pH 5.5, 40°C Xanthomonas citri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.100 99480
-
calculation from sequence Xanthomonas citri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.100 Xanthomonas citri Q8PI23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.100
-
Xanthomonas citri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.100 4-nitrophenyl beta-D-mannopyranoside + H2O
-
Xanthomonas citri 4-nitrophenol + beta-D-mannopyranoside
-
?
3.2.1.100 beta-mannan + H2O
-
Xanthomonas citri D-mannose + ?
-
?
3.2.1.100 galactomannan + H2O
-
Xanthomonas citri D-mannose + ?
-
?
3.2.1.100 mannobiose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri mannose +
-
?
3.2.1.100 mannohexaose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
?
3.2.1.100 mannopentaose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
?
3.2.1.100 mannotetraose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri D-mannose + ?
-
?
3.2.1.100 mannotriose + H2O increasing hydrolysis rates are observed from mannobiose to mannohexaose, indicating the preference for longer manno-oligosaccharides Xanthomonas citri mannobiose + D-mannose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.100 exo-beta-mannanase
-
Xanthomonas citri
3.2.1.100 XacMan2A
-
Xanthomonas citri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.100 40
-
-
Xanthomonas citri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.100 20 50 20°C: about 40% of maximal activity, 50°C: about 40% of maximal activity Xanthomonas citri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.100 40
-
4 h, about 50% loss of activity Xanthomonas citri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.100 12.53
-
beta-mannan pH 5.5, 40°C Xanthomonas citri
3.2.1.100 126.86
-
mannohexaose pH 5.5, 40°C Xanthomonas citri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.100 5.5
-
-
Xanthomonas citri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.100 5 7 pH 5.0: about 70% of maximal activity, pH 7.0: about 45% of maximal activity Xanthomonas citri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.100 2.98
-
beta-mannan pH 5.5, 40°C Xanthomonas citri
3.2.1.100 24.82
-
mannohexaose pH 5.5, 40°C Xanthomonas citri