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Literature summary extracted from

  • Mine, S.; Watanabe, M.; Kamachi, S.; Abe, Y.; Ueda, T.
    The structure of an archaeal beta-glucosaminidase provides insight into glycoside hydrolase evolution (2017), J. Biol. Chem., 292, 4996-5006 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expressed in Escherichia coli Rosetta (DE3)pLysS cells Pyrococcus horikoshii
3.2.1.165 expressed in Escherichia coli Rosetta (DE3)pLysS cells Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.165 in complex with D-glucosamine, sitting drop vapor diffusion method, using in 100 mM Tris-HCl (pH 8.5) and 20% (w/v) polyethylene glycol 1000 Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.165 D178N inactive Thermococcus kodakarensis
3.2.1.165 D180N inactive Pyrococcus horikoshii
3.2.1.165 E179Q the mutant shows 3% of wild type activity Thermococcus kodakarensis
3.2.1.165 E181Q the mutant shows 10% of wild type activity Pyrococcus horikoshii
3.2.1.165 E181Q the mutant shows 15% of wild type activity Pyrococcus horikoshii
3.2.1.165 E306Q the mutant shows 40% of wild type activity Thermococcus kodakarensis
3.2.1.165 E347Q inactive Thermococcus kodakarensis
3.2.1.165 E349Q inactive Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Pyrococcus horikoshii
-
-
-
3.2.1.165 Thermococcus kodakarensis Q76HN4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 Ni-NTA column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration Pyrococcus horikoshii
3.2.1.165 Ni-NTA column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitobiose + H2O
-
 Pyrococcus horikoshii 2 D-glucosamine
-
 ?
3.2.1.165 chitobiose + H2O
-
 Thermococcus kodakarensis 2 D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Pyrococcus horikoshii
3.2.1.165 exo-beta-D-glucosaminidase
-
 Thermococcus kodakarensis
3.2.1.165 GlmA
-
 Pyrococcus horikoshii
3.2.1.165 GlmA
-
 Thermococcus kodakarensis