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Literature summary extracted from

  • Gorelik, A.; Heinz, L.X.; Illes, K.; Superti-Furga, G.; Nagar, B.
    Crystal structure of the acid sphingomyelinase-like phosphodiesterase SMPDL3B provides insights into determinants of substrate specificity (2016), J. Biol. Chem., 291, 24054-24064 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in Sf9 cells and RAW264.7 cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.12 apo-enzyme and in complex with phosphorylcholine, hanging drop vapor diffusion method, using 0.2 M NH4NO3 and 25% (w/v) PEG 3350 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 H135A almost inactive Mus musculus
3.1.4.12 K140M the mutant shows reduced activity compared to the wild type enzyme Mus musculus
3.1.4.12 K140M/N141A almost inactive Mus musculus
3.1.4.12 N141A the mutant shows reduced activity compared to the wild type enzyme Mus musculus
3.1.4.12 N200A the mutant shows increased activity compared to the wild type enzyme Mus musculus
3.1.4.12 Y198F the mutant shows reduced activity compared to the wild type enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 cell surface
-
 Mus musculus 9986
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ contains two Zn2+ ions Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Mus musculus P58242
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 ADP + H2O
-
 Mus musculus AMP + phosphate
-
 ?
3.1.4.12 ATP + H2O
-
 Mus musculus ADP + phosphate
-
 ?
3.1.4.12 bis(4-nitrophenyl)phosphate + H2O
-
 Mus musculus ?
-
 ?
3.1.4.12 CDP-choline + H2O
-
 Mus musculus CMP + phosphorylcholine
-
 ?
3.1.4.12 additional information no activity with AMP and diadenosine tetraphosphate Mus musculus ?
-
 ?
3.1.4.12 additional information the soluble enzyme form that lacks the GPI moiety responsible for anchoring SMPDL3B to the cell surface displays no head group hydrolysis with the following substrates: sphingomyelin in Triton X-100 micelles, sphingomyelin in neutral liposomes (also comprising phosphatidylcholine and cholesterol), the soluble micelle-forming lipids sphingosylphosphorylcholine, and platelet-activating factor Mus musculus ?
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Mus musculus ceramide + phosphorylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 acid sphingomyelinase-like phosphodiesterase 3B
-
 Mus musculus
3.1.4.12 SMPDL3B
-
 Mus musculus

General Information

EC Number General Information Comment Organism
3.1.4.12 physiological function the enzyme acts as a negative regulator of innate immune signaling, affecting cellular lipid composition and membrane fluidity Mus musculus