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Literature summary extracted from

  • Chen, D.; Vollmar, M.; Rossi, M.N.; Phillips, C.; Kraehenbuehl, R.; Slade, D.; Mehrotra, P.V.; von Delft, F.; Crosthwaite, S.K.; Gileadi, O.; Denu, J.M.; Ahel, I.
    Identification of macrodomain proteins as novel O-acetyl-ADP-ribose deacetylases (2011), J. Biol. Chem., 286, 13261-13271 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.106 enzyme isoform MacroD1 is expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.106 isoform MacroD1, sitting drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350 at 4°C Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.106 C199A the mutant shows about 90.4% of wild type activity Homo sapiens
3.1.1.106 D122A the mutant shows about 24.5% of wild type activity Staphylococcus aureus
3.1.1.106 D160A the mutant shows about 57% of wild type activity Homo sapiens
3.1.1.106 D167A the mutant shows about 68.7% of wild type activity Homo sapiens
3.1.1.106 D184A the mutant shows about 39.3% of wild type activity Homo sapiens
3.1.1.106 G124E the mutant shows about 1.0% of wild type activity Escherichia coli
3.1.1.106 G214E the mutant shows about 0.2% of wild type activity Staphylococcus aureus
3.1.1.106 G270E the mutant shows about 1% of wild type activity Homo sapiens
3.1.1.106 H188A the mutant shows about 57.6% of wild type activity Homo sapiens
3.1.1.106 N171A the mutant shows about 41.3% of wild type activity Homo sapiens
3.1.1.106 N174A the mutant shows about 57.3% of wild type activity Homo sapiens
3.1.1.106 N174A/D184A the mutant shows about 7% of wild type activity Homo sapiens
3.1.1.106 S176A the mutant shows about 98.4% of wild type activity Homo sapiens
3.1.1.106 S268A the mutant shows about 77% of wild type activity Homo sapiens
3.1.1.106 T226A the mutant shows about 102.9% of wild type activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.106 ADP-D-ribose
-
Escherichia coli
3.1.1.106 ADP-D-ribose
-
Homo sapiens
3.1.1.106 ADP-D-ribose
-
Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.106 0.107
-
O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.27
-
O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Escherichia coli
3.1.1.106 0.375
-
O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 2
-
O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O Staphylococcus aureus
-
ADP-D-ribose + acetate
-
?
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O Escherichia coli
-
ADP-D-ribose + acetate
-
?
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O Staphylococcus aureus
-
ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O Escherichia coli
-
ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
ADP-D-ribose + acetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.106 Escherichia coli
-
-
-
3.1.1.106 Homo sapiens A1Z1Q3
-
-
3.1.1.106 Homo sapiens Q9BQ69
-
-
3.1.1.106 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.106 HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.106 HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O
-
Staphylococcus aureus ADP-D-ribose + acetate
-
?
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O
-
Escherichia coli ADP-D-ribose + acetate
-
?
3.1.1.106 2''-O-acetyl-ADP-D-ribose + H2O
-
Homo sapiens ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O
-
Staphylococcus aureus ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O
-
Escherichia coli ADP-D-ribose + acetate
-
?
3.1.1.106 3''-O-acetyl-ADP-D-ribose + H2O
-
Homo sapiens ADP-D-ribose + acetate
-
?
3.1.1.106 O-acetyl-ADP-D-ribose + H2O
-
Staphylococcus aureus ADP-D-ribose + acetate
-
?
3.1.1.106 O-acetyl-ADP-D-ribose + H2O
-
Escherichia coli ADP-D-ribose + acetate
-
?
3.1.1.106 O-acetyl-ADP-D-ribose + H2O
-
Homo sapiens ADP-D-ribose + acetate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.106 MacroD-like protein
-
Staphylococcus aureus
3.1.1.106 MACROD1
-
Homo sapiens
3.1.1.106 MacroD2
-
Homo sapiens
3.1.1.106 O-acetyl-ADP-ribose deacetylase
-
Staphylococcus aureus
3.1.1.106 O-acetyl-ADP-ribose deacetylase
-
Escherichia coli
3.1.1.106 O-acetyl-ADP-ribose deacetylase
-
Homo sapiens
3.1.1.106 OAADPr deacetylase
-
Staphylococcus aureus
3.1.1.106 OAADPr deacetylase
-
Escherichia coli
3.1.1.106 OAADPr deacetylase
-
Homo sapiens
3.1.1.106 SAV0325
-
Staphylococcus aureus
3.1.1.106 ymdB
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.106 0.002
-
O-acetyl-ADP-D-ribose mutant G270E of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.005
-
O-acetyl-ADP-D-ribose mutant G124E, at pH 7.3 and 23°C Escherichia coli
3.1.1.106 0.007
-
O-acetyl-ADP-D-ribose mutant G214E, at pH 7.3 and 23°C Staphylococcus aureus
3.1.1.106 0.014
-
O-acetyl-ADP-D-ribose mutant N174A/D184A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.078
-
O-acetyl-ADP-D-ribose mutant D184A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.082
-
O-acetyl-ADP-D-ribose mutant N171A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.113
-
O-acetyl-ADP-D-ribose mutant N174A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.114
-
O-acetyl-ADP-D-ribose mutant D160A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.114
-
O-acetyl-ADP-D-ribose mutant H188A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.12
-
O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.136
-
O-acetyl-ADP-D-ribose mutant D167A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.153
-
O-acetyl-ADP-D-ribose mutant S268A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.179
-
O-acetyl-ADP-D-ribose mutant C199A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.195
-
O-acetyl-ADP-D-ribose mutant S176A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.198
-
O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.204
-
O-acetyl-ADP-D-ribose mutant T226A of isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 0.48
-
O-acetyl-ADP-D-ribose wild type enzyme, at pH 7.3 and 23°C Escherichia coli
3.1.1.106 0.679
-
O-acetyl-ADP-D-ribose mutant D122A, at pH 7.3 and 23°C Staphylococcus aureus
3.1.1.106 2.77
-
O-acetyl-ADP-D-ribose wild type enzyme, at pH 7.3 and 23°C Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.106 0.145
-
ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.106 0.533
-
O-acetyl-ADP-D-ribose isoform MacroD1, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 1.1
-
O-acetyl-ADP-D-ribose isoform MacroD2, at pH 7.3 and 23°C Homo sapiens
3.1.1.106 1.78
-
O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Escherichia coli
3.1.1.106 1.84
-
O-acetyl-ADP-D-ribose at pH 7.3 and 23°C Staphylococcus aureus