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Literature summary extracted from

  • Rahier, A.; Darnet, S.; Bouvier, F.; Camara, B.; Bard, M.
    Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana (2006), J. Biol. Chem., 281, 27264-27277 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.418 expressed in Saccharomyces cerevisiae strain erg26 SDG200 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.418 0.0031
-
 NAD+ isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.008
-
 NAD+ isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.081
-
 3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.087
-
 4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.112
-
 3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.134
-
 4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.204
-
 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.274
-
 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.383
-
 4alpha-carboxy-cholest-7-en-3alpha-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
1.1.1.418 0.655
-
 4alpha-carboxy-cholest-7-en-3alpha-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.418 microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.418 a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+ Arabidopsis thaliana
-
 a 3-oxosteroid + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.418 Arabidopsis thaliana Q67ZE1
-
-
1.1.1.418 Arabidopsis thaliana Q9FX01
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.418 3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
-
 Arabidopsis thaliana ? + CO2 + NADH + H+
-
 ?
1.1.1.418 4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cycloergost-24(241)-en-3beta-ol + NAD+
-
 Arabidopsis thaliana 4alpha,14alpha-dimethyl-9beta,19-cyclo-ergost-24(241)-en-3-one + CO2 + NADH + H+
-
 ?
1.1.1.418 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
-
 Arabidopsis thaliana 4alpha-methyl-cholest-8,24-dien-3-one + CO2 + NADH + H+
-
 ?
1.1.1.418 4alpha-carboxy-cholest-7-en-3alpha-ol + NAD+
-
 Arabidopsis thaliana cholest-7-en-3-one + CO2 + NADH + H+
-
 ?
1.1.1.418 4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
-
 Arabidopsis thaliana cholest-7-en-3-one + CO2 + NADH + H+
-
 ?
1.1.1.418 4alpha-carboxy-stigmasta-7,24(241)-dien-3beta-ol + NAD+
-
 Arabidopsis thaliana stigmasta-7,24(241)-dien-3-one + CO2 + NADH + H+
-
 ?
1.1.1.418 a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+
-
 Arabidopsis thaliana a 3-oxosteroid + CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.418 3beta-hydroxysteroid dehydrogenase/C-4 decarboxylase
-
 Arabidopsis thaliana
1.1.1.418 3betaHSD/D1 isoform Arabidopsis thaliana
1.1.1.418 3betaHSD/D2 isoform Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.418 NAD+
-
 Arabidopsis thaliana