EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.10 | cyanide | - |
Zea mays | |
1.14.18.10 | cytochrome c | - |
Zea mays | |
1.14.18.10 | menadione | 39% residual activity at 0.05 mM | Zea mays | |
1.14.18.10 | additional information | not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis | Zea mays | |
1.14.18.10 | orthophenanthroline | 44% residual activity at 1 mM | Zea mays | |
1.14.18.11 | cyanide | - |
Zea mays | |
1.14.18.11 | cytochrome c | - |
Zea mays | |
1.14.18.11 | menadione | 34% residual activity at 0.05 mM | Zea mays | |
1.14.18.11 | additional information | not inhibited by N3-, CO (saturated), LAB 170250F, and tetcyclacis | Zea mays | |
1.14.18.11 | orthophenanthroline | 47% residual activity at 1 mM | Zea mays | |
1.14.18.11 | stigmastan-3beta,5alpha,6alpha-triol | effectively inhibited by stigmastan-3beta,5alpha,6alpha-triol | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.18.10 | microsome | - |
Zea mays | - |
- |
1.14.18.11 | microsome | - |
Zea mays | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.10 | 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | Zea mays | overall reaction | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.10 | Zea mays | - |
- |
- |
1.14.18.11 | Zea mays | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.10 | 24(25)-dihydrocycloartenol + NAD(P)H + H+ | 30% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
1.14.18.10 | 24-methylidenecycloartanol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | overall reaction | Zea mays | 3beta-hydroxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-ergost-24(241)-ene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O | - |
? | |
1.14.18.10 | 24-methylidenecycloartanol + NAD(P)H + O2 + H+ | 100% activity | Zea mays | 4alpha-hydroxymethyl-4beta,14alpha-dimethyl-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD(P)+ + H2O | - |
? | |
1.14.18.10 | 24zeta-methylcycloartanol + NAD(P)H + H+ | 43% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
1.14.18.10 | cyclolaudenol + NAD(P)H + H+ | 56% activity compared to 24-methylidenecycloartanol | Zea mays | ? | - |
? | |
1.14.18.10 | additional information | no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol | Zea mays | ? | - |
? | |
1.14.18.11 | 24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ | - |
Zea mays | (3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O | - |
ir | |
1.14.18.11 | 24-ethylidenelophenol + NAD(P)H + O2 + H+ | 100% activity | Zea mays | ? | - |
? | |
1.14.18.11 | 24-methylidenelophenol + NAD(P)H + H+ | 59% activity compared to 24-ethylidenelophenol | Zea mays | ? | - |
? | |
1.14.18.11 | 24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+ | 10% activity compared to 24-ethylidenelophenol | Zea mays | ? | - |
? | |
1.14.18.11 | cycloeucalenol + NAD(P)H + H+ | 10% activity compared to 24-ethylidenelophenol | Zea mays | ? | - |
? | |
1.14.18.11 | lophenol + NAD(P)H + H+ | 38% activity compared to 24-ethylidenelophenol | Zea mays | ? | - |
? | |
1.14.18.11 | additional information | no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.10 | 4,4-dimethylsterol-oxidase | - |
Zea mays |
1.14.18.11 | 4alpha-methyl oxidase | - |
Zea mays |
1.14.18.11 | 4alpha-methylsterol C4 oxidase | - |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.10 | NADH | required | Zea mays | |
1.14.18.10 | NADPH | required | Zea mays | |
1.14.18.11 | NADH | required | Zea mays | |
1.14.18.11 | NADPH | required | Zea mays |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.14.18.10 | 0.01 | - |
using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication | Zea mays | cyanide | |
1.14.18.10 | 0.03 | - |
using 24-methylenecycloartanol as substrate, at 30°C, pH not specified in the publication | Zea mays | cytochrome c | |
1.14.18.11 | 0.035 | - |
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication | Zea mays | cytochrome c | |
1.14.18.11 | 0.05 | - |
using 24-ethylidenelophenol as substrate, at 30°C, pH not specified in the publication | Zea mays | cyanide |