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Literature summary extracted from

  • Sims, J.; Capon, D.; Dressler, D.
    dnaG (primase)-dependent origins of DNA replication. Nucleotide sequences of the negative strand initiation sites of bacteriophages St-1, phiK, and alpha3 (1979), J. Biol. Chem., 254, 12615-12628 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.101 Escherichia coli
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.101 bacteriophage alpha3 ssDNA + n rNTP
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 Escherichia coli bacteriophage alpha3 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
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2.7.7.101 bacteriophage G4 ssDNA + n rNTP
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 Escherichia coli bacteriophage G4 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
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2.7.7.101 bacteriophage phiK ssDNA + n rNTP
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 Escherichia coli bacteriophage phiK ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
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 ?
2.7.7.101 bacteriophage ST-1 ssDNA + n rNTP
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 Escherichia coli bacteriophage ST-1 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
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 ?
2.7.7.101 additional information in all four phages studied, the negative strand initiation site occurs within an intercistronic region of approximately 135 bases. Extensive nucleotide conservation exists at the negative strand origin. The conserved origin DNA occurs in two regions, 42 and 45 bases long, which are separated by 13 bases of divergent sequence. The start point of negative strand synthesis lies just prior to one of these hairpins correlated with the two stretches of conserved nucleotide sequence Escherichia coli ?
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