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Literature summary extracted from

  • Bai, Y.; Yang, J.; Eisele, L.E.; Underwood, A.J.; Koestler, B.J.; Waters, C.M.; Metzger, D.W.; Bai, G.
    Two DHH subfamily 1 proteins in Streptococcus pneumoniae possess cyclic di-AMP phosphodiesterase activity and affect bacterial growth and virulence (2013), J. Bacteriol., 195, 5123-5132 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.59 0.0167
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 7.5, 37°C Streptococcus pneumoniae D39
3.1.4.59 0.0364
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 7.5, 37°C Streptococcus pneumoniae D39
3.1.4.60 0.0239
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 7.5, 37°C Streptococcus pneumoniae D39

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.59 membrane protein possesses two transmembrane helices Streptococcus pneumoniae D39 16020
-
3.1.4.60 membrane protein possesses two transmembrane helices Streptococcus pneumoniae D39 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.59 Mn2+ required Streptococcus pneumoniae D39
3.1.4.60 Mn2+ required Streptococcus pneumoniae D39

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.59 Streptococcus pneumoniae D39 A0A0H2ZNP2
-
-
3.1.4.59 Streptococcus pneumoniae D39 A0A0H2ZQZ4
-
-
3.1.4.60 Streptococcus pneumoniae D39 A0A0H2ZNP2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.59 5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
 Streptococcus pneumoniae D39 2 AMP
-
 ?
3.1.4.59 cyclic di-3',5'-adenylate + H2O
-
 Streptococcus pneumoniae D39 5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
 ?
3.1.4.59 additional information Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine Streptococcus pneumoniae D39 ?
-
 ?
3.1.4.60 5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
 Streptococcus pneumoniae D39 2 AMP
-
 ?
3.1.4.60 cyclic di-3',5'-adenylate + H2O
-
 Streptococcus pneumoniae D39 5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
 ?
3.1.4.60 additional information Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine Streptococcus pneumoniae D39 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.59 dimer 2 * 35000, calculated from sequence Streptococcus pneumoniae D39
3.1.4.59 additional information protein possesses two transmembrane helices, a PAS domain, an atypical GGDEF domain, a DHH domain, and a DHHA1 domain Streptococcus pneumoniae D39
3.1.4.60 dimer 2 * 35000, calculated from sequence Streptococcus pneumoniae D39
3.1.4.60 additional information protein possesses two transmembrane helices, a PAS domain, an atypical GGDEF domain, a DHH domain, and a DHHA1 domain Streptococcus pneumoniae D39

Synonyms

EC Number Synonyms Comment Organism
3.1.4.59 PDE1
-
 Streptococcus pneumoniae D39
3.1.4.59 PDE2
-
 Streptococcus pneumoniae D39
3.1.4.59 SPD_1153
-
 Streptococcus pneumoniae D39
3.1.4.59 SPD_2032
-
 Streptococcus pneumoniae D39
3.1.4.60 PDE2
-
 Streptococcus pneumoniae D39
3.1.4.60 SPD_1153
-
 Streptococcus pneumoniae D39

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.59 8.5
-
-
 Streptococcus pneumoniae D39
3.1.4.60 8.5
-
-
 Streptococcus pneumoniae D39

General Information

EC Number General Information Comment Organism
3.1.4.59 physiological function deletion of either isoform Pde1 or Pde2 results in a moderate increase of the c-di-AMP levels compared with the parental strain. Deletion of both genes results in an up to 4fold increase in c-di-AMP levels compared to that of the parental strain. Both Pde1 and Pde2 play a role in pneumococcal growth. Deletion of either isoform Pde1 or Pde2 reduces the growth rate slightly, and the double mutant synergizes the reduction Streptococcus pneumoniae D39
3.1.4.60 physiological function deletion of either isoform Pde1 or Pde2 results in a moderate increase of the c-di-AMP levels compared with the parental strain. Deletion of both genes results in an up to 4fold increase in c-di-AMP levels compared to that of the parental strain. Both Pde1 and Pde2 play a role in pneumococcal growth. Deletion of either isoform Pde1 or Pde2 reduces the growth rate slightly, and the double mutant synergizes the reduction Streptococcus pneumoniae D39