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Literature summary extracted from

  • Thirlway, J.; Soultanas, P.
    In the Bacillus stearothermophilus DnaB-DnaG complex, the activities of the two proteins are modulated by distinct but overlapping networks of residues (2006), J. Bacteriol., 188, 1534-1539 .
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.101 Geobacillus stearothermophilus Q9X4D0
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.101 additional information enzyme initiates at 3'-ATC-5' and 3'-ATT-5' sites synthesizing primers that are 22 or 23 nucleotides long Geobacillus stearothermophilus ?
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2.7.7.101 ssDNA + n NTP
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 Geobacillus stearothermophilus ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate
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General Information

EC Number General Information Comment Organism
2.7.7.101 physiological function in the presence of the helicase DnaB the size distribution of primers is different, and a range of additional smaller primers are also synthesized. Mutations E15A, Y88A, and E15A Y88A in DnaB bind DnaG but are not able to modulate primer size, whereas the R195A/M196A mutant inhibits the primase activity Geobacillus stearothermophilus