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Literature summary extracted from

  • York, G.M.; Lupberger, J.; Tian, J.; Lawrence, A.G.; Stubbe, J.; Sinskey, A.J.
    Ralstonia eutropha H16 encodes two and possibly three intracellular poly[D-(-)-3-hydroxybutyrate] depolymerase genes (2003), J. Bacteriol., 185, 3788-3794 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.22 gene phaZ1, pha sequences comparisons, subcloning in Escherichia coli strains DH5alpha and S17-1 Cupriavidus necator
3.1.1.22 gene phaZ2, pha sequences comparisons, subcloning in strains DH5alpha and S17-1 Cupriavidus necator
3.1.1.75 gene phaZ3, pha sequences comparisons, subcloning in strains DH5alpha and S17-1 Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.22 additional information generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate) Cupriavidus necator
3.1.1.75 additional information generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate) Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 intracellular
-
 Cupriavidus necator 5622
-
3.1.1.22 additional information the enzyme sequence contains no signal sequence Cupriavidus necator
-
-
3.1.1.75 intracellular
-
 Cupriavidus necator 5622
-
3.1.1.75 additional information the enzyme sequence contains no signal sequence Cupriavidus necator
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Cupriavidus necator O87189 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-
3.1.1.22 Cupriavidus necator Q7WT49 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-
3.1.1.75 Cupriavidus necator Q7WT48 i.e. Alcaligenes eutrophus or Ralstonia eutropha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 additional information enzyme PhaZ1 converts the polymer into oligomers and monomers of hydroxybutyrate by the end of the degradation process Cupriavidus necator ?
-
 ?
3.1.1.75 [(R)-3-hydroxybutanoate]n + H2O x is 1-5 Cupriavidus necator [(R)-3-hydroxybutanoate]n-x + [(R)-3-hydroxybutanoate]x
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.22 intracellular PHB depolymerase UniProt Cupriavidus necator
3.1.1.22 More cf. EC 3.1.1.75 Cupriavidus necator
3.1.1.22 PhaZ
-
 Cupriavidus necator
3.1.1.22 PhaZ1
-
 Cupriavidus necator
3.1.1.22 PhaZ2
-
 Cupriavidus necator
3.1.1.22 phaZre
-
 Cupriavidus necator
3.1.1.22 poly[D-(-)-3-hydroxybutyrate] depolymerase
-
 Cupriavidus necator
3.1.1.75 intracellular poly(3-hydroxybutyrate) depolymerase UniProt Cupriavidus necator
3.1.1.75 phaZ3
-
 Cupriavidus necator

General Information

EC Number General Information Comment Organism
3.1.1.22 additional information enzyme PhaZ1 has neither a lipase box nor an identifiable PHB binding domain Cupriavidus necator