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Literature summary extracted from

  • Xun, L.; Topp, E.; Orser, C.
    Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp (1992), J. Bacteriol., 174, 8003-8007 .
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.4.5 additional information optimal activity at 0.1 mM total salts Sphingobium chlorophenolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.5 60000
-
 non-denaturing PAGE Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.5 2,3,5,6-tetrachlorohydroquinone + 2 glutathione Sphingobium chlorophenolicum the enzyme is involved in degradation of pentachlorophenol 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,6-trichlorohydroquinone + 2 glutathione Sphingobium chlorophenolicum the enzyme is involved in degradation of pentachlorophenol 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.5 Sphingobium chlorophenolicum Q03520
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.21.4.5 sensitive to oxygen, a loss of enzyme activity occurrs when DTT is not included in elution buffers during purification Sphingobium chlorophenolicum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.5
-
 Sphingobium chlorophenolicum

Storage Stability

EC Number Storage Stability Organism
1.21.4.5 -20°C, no measurable loss of activity over a period of 1 month Sphingobium chlorophenolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.5 2,3,5,6-tetrachlorohydroquinone + 2 glutathione the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,5,6-tetrachlorohydroquinone + 2 glutathione the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent Sphingobium chlorophenolicum 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,6-trichlorohydroquinone + 2 glutathione the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,6-trichlorohydroquinone + 2 glutathione the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent Sphingobium chlorophenolicum 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.5 dimer 2 * 30000, SDS-PAGE Sphingobium chlorophenolicum

Synonyms

EC Number Synonyms Comment Organism
1.21.4.5 PCP hydroxylase
-
 Sphingobium chlorophenolicum
1.21.4.5 tetrachloro-p-hydroquinone reductive dehalogenase
-
 Sphingobium chlorophenolicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.4.5 40
-
-
 Sphingobium chlorophenolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.4.5 6 7
-
 Sphingobium chlorophenolicum

General Information

EC Number General Information Comment Organism
1.21.4.5 metabolism the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum