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Literature summary extracted from
- Discovery and characterization of a novel chitosanase from Paenibacillus dendritiformis by phylogeny-based enzymatic product specificity prediction (2018), J. Agric. Food Chem., 66, 4645-4651 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | synthesis | the enzyme is a good candidate for production of beta-D-GlcN-(1->4)-beta-D-GlcN | Paenibacillus dendritiformis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.132 | expressed in Escherichia coli BL21 (DE3) | Paenibacillus dendritiformis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 31000 | - |
SDS-PAGE | Paenibacillus dendritiformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.132 | Paenibacillus dendritiformis | A0A2W4GVN6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.132 | - |
Paenibacillus dendritiformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.132 | chitosan + H2O | colloidal chitosan, endotype cleavage pattern and hydrolysis of chitosan to yield (GlcN)2 as the major product | Paenibacillus dendritiformis | beta-D-GlcN-(1->4)-beta-D-GlcN + ? | - |
? |  |
| 3.2.1.132 | additional information | the enzyme is not active toward colloidal chitin or carboxymethyl cellulose | Paenibacillus dendritiformis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | ? | x * 31000, SDS-PAGE | Paenibacillus dendritiformis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | Csn-PD | - |
Paenibacillus dendritiformis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 45 | - |
- |
Paenibacillus dendritiformis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 20 | 55 | 20°C: about 75% of maximal activity, 55°C: about 70% of maximal activity | Paenibacillus dendritiformis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 60 | - |
30 min, 90% loss of activity | Paenibacillus dendritiformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 7 | - |
- |
Paenibacillus dendritiformis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 4 | 7.5 | pH 4.0: about 50% of maximal activity, pH 7.5: about 40% of maximal activity | Paenibacillus dendritiformis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 6 | 7 | stable | Paenibacillus dendritiformis |
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Release 2023.1 (January 2023)
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