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Literature summary extracted from
- Synthesis of isomalto-oligosaccharides by Pichia pastoris displaying the Aspergillus niger alpha-glucosidase (2017), J. Agric. Food Chem., 65, 9468-9474 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | gene aglU, recombinant expression from plasmid pKANGL-GCW61 in Pichia pastoris strain GS115, cell surface display, recombinant expression in Escherichia coli TOP10 cells | Aspergillus niger |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | additional information | preparation of the AglU-displaying Pichia pastoris whole-cell biocatalyst for optimized synthesis of isomaltooligosaccharides (IMOs). IMOs are a mixture that includes isomaltose (IG2), panose (P), and isomaltotriose (IG3). The recombinant Aspergillus niger enzyme expressed from Pichia pastoris has a high reaction efficiency and operational stability, method optimization and evaluation, overview. IMOs synthesis by transglycosylation, best at pH 4.0 and 55-60°C | Aspergillus niger |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.20 | extracellular | - |
Aspergillus niger | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Aspergillus niger | A2QJF7 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | maltose + H2O | - |
Aspergillus niger | 2 D-glucose | - |
? |  |
| 3.2.1.20 | additional information | the recombinant enzyme performs isomaltooligosaccharides (IMOs) synthesis by transglycosylation. Quantitative analyses of products maltose (M), isomaltose (IG2), panose (P), and isomaltotriose (IG3) | Aspergillus niger | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | aGlu | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 55 | - |
assay at | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 5 | - |
assay at | Aspergillus niger |
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Release 2023.1 (January 2023)
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