Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bashirova, A.; Pramanik, S.; Volkov, P.; Rozhkova, A.; Nemashkalov, V.; Zorov, I.; Gusakov, A.; Sinitsyn, A.; Schwaneberg, U.; Davari, M.D.
    Disulfide bond engineering of an endoglucanase from Penicillium verruculosum to improve its thermostability (2019), Int. J. Mol. Sci., 20, E1602 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information molecular dynamics simulations reveal that the introduced disulfide bond rigidified a global structure of DSB2 and DSB3 mutant variants, thus enhancing their thermostability Talaromyces verruculosus
3.2.1.4 S127C/A165C site-directed mutagenesis, introduction of a disulfide bond into mutant DSB2, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII Talaromyces verruculosus
3.2.1.4 Y171C/L201C site-directed mutagenesis, introduction of a disulfide bond into mutant DSB3, the mutant enzyme displays 15-21% increased specific activity against carboxymethylcellulose and beta-glucan, and increased thermostability compared to wild-type enzyme EGLII Talaromyces verruculosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Talaromyces verruculosus A0A1U7Q1U3 i.e. Penicillium verruculosum
-
3.2.1.4 Talaromyces verruculosus ATCC 10513 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
3.2.1.4 Talaromyces verruculosus CBS 388.48 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
3.2.1.4 Talaromyces verruculosus DSM 2263 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
3.2.1.4 Talaromyces verruculosus IMI 040039 A0A1U7Q1U3 i.e. Penicillium verruculosum
-
3.2.1.4 Talaromyces verruculosus NRRL 1050 A0A1U7Q1U3 i.e. Penicillium verruculosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 53
-
 purified recombinant wild-type enzyme, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
3.2.1.4 57
-
 purified recombinant wild-type enzyme, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus
3.2.1.4 61
-
 purified recombinant mutant S127C/A165C, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
3.2.1.4 64
-
 purified recombinant mutant Y171C/L201C, substrate beta-glucan, pH and temperature not specified in the publication Talaromyces verruculosus
3.2.1.4 66
-
 purified recombinant mutant Y171C/L201C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus
3.2.1.4 68
-
 purified recombinant mutant S127C/A165C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication Talaromyces verruculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus DSM 2263 ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus NRRL 1050 ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus CBS 388.48 ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus ATCC 10513 ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Talaromyces verruculosus IMI 040039 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus DSM 2263 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus NRRL 1050 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus CBS 388.48 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus ATCC 10513 ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Talaromyces verruculosus IMI 040039 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EGLII
-
 Talaromyces verruculosus
3.2.1.4 endoglucanase
-
 Talaromyces verruculosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 70
-
 2 h, the wild-type enzyme retains 38% activity, the enzyme mutants retain 52% and 58% activity, respectively Talaromyces verruculosus
3.2.1.4 80
-
 2 h, the wild-type enzyme retains no activity, the enzyme mutants retain 15% and 22% activity, respectively Talaromyces verruculosus

General Information

EC Number General Information Comment Organism
3.2.1.4 additional information catalytic residues are E142 and E249 in the enzyme active site. The enzyme contains three potential disulfide bonds Talaromyces verruculosus