Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dong, B.; Luo, H.; Liu, B.; Li, W.; Ou, S.; Wu, Y.; Zhang, X.; Pang, X.; Zhang, Z.
    BcXyl, a beta-xylosidase isolated from Brunfelsia calycina flowers with anthocyanin-beta-glycosidase activity (2019), Int. J. Mol. Sci., 20, 1423 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37
-
 Brunfelsia pauciflora var. calycina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ 1 mM, 88% inhibition Brunfelsia pauciflora var. calycina
3.2.1.37 D-gluconate 1 mM, 87% inhibition Brunfelsia pauciflora var. calycina
3.2.1.37 Hg2+ 1 mM, 40% inhibition Brunfelsia pauciflora var. calycina
3.2.1.37 additional information 10 mM Mg2+, Cu2+, Mn2+ and Ca2+ show no inhibition Brunfelsia pauciflora var. calycina

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Brunfelsia pauciflora var. calycina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Brunfelsia pauciflora var. calycina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 flower
-
 Brunfelsia pauciflora var. calycina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Brunfelsia pauciflora var. calycina 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 39.36% of the activitzy compared to 4-nitrophenyl beta-D-galactopyranoside Brunfelsia pauciflora var. calycina 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 anthocyanin + H2O the enzyme catalyzes the degradation of an anthocyanin component of Brunfelsia calycina, and the release of xylose and galactose due to hydrolysis of glycosidic bonds by BcXyl is detected Brunfelsia pauciflora var. calycina ?
-
 ?
3.2.1.37 additional information no activity to 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl-beta-D-mannopyranoside. Based on fragment sequences of the enzyme detected by tandem mass spectrometry and their identical hits from other plant species the purified enzyme is a beta-xylosidase Brunfelsia pauciflora var. calycina ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 BcXyl
-
 Brunfelsia pauciflora var. calycina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 40
-
-
 Brunfelsia pauciflora var. calycina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 30 50 30°C: about 55% of maximal activity, 50°C: about 75% of maximal activity Brunfelsia pauciflora var. calycina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
-
 Brunfelsia pauciflora var. calycina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4 6 pH 4.0: about 45% of maximal activity, pH 6.0: about 62% of maximal activity Brunfelsia pauciflora var. calycina

General Information

EC Number General Information Comment Organism
3.2.1.37 physiological function the enzyme catalyzes the degradation of an anthocyanin component of Brunfelsia calycina, and the release of xylose and galactose due to hydrolysis of glycosidic bonds by BcXyl is detected. The enzyme is activated at the stage at which the rapid anthocyanin degradation occurrs Brunfelsia pauciflora var. calycina