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Literature summary extracted from

  • Huang, R.; Zhong, L.; Xie, F.; Wei, L.; Gan, L.; Wang, X.; Liao, A.
    Purification, characterization and degradation performance of a novel dextranase from Penicillium cyclopium CICC-4022 (2019), Int. J. Mol. Sci., 20, 1360 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.11 SDS 106.16% activity at 1 mM Penicillium cyclopium
3.2.1.11 Tris 103.79% activity at 1 mM Penicillium cyclopium
3.2.1.11 Urea 112.79% activity at 1 mM Penicillium cyclopium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 Ca2+ 40.73% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 Cr2+ 48.25% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 Cu2+ 16.41% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 EDTA 80.58% residual activity at 5 mM Penicillium cyclopium
3.2.1.11 Fe3+ 64.61% residual activity at 5 mM Penicillium cyclopium
3.2.1.11 Ni2+ 67.71% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 Pb2+ 20.39% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 SDS 34.15% residual activity at 10 mM Penicillium cyclopium
3.2.1.11 Zn2+ 16.85% residual activity at 10 mM Penicillium cyclopium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 2.45
-
 dextran T2000 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 2.61
-
 dextran T70 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 2.72
-
 dextran T5 at pH 5.0 and 50°C Penicillium cyclopium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.11 Fe2+ 109.95% activity at 10 mM Penicillium cyclopium
3.2.1.11 Li+ 116.28% activity at 1 mM Penicillium cyclopium
3.2.1.11 Na+ 111.48% activity at 5 mM Penicillium cyclopium
3.2.1.11 NH4+ 107.58% activity at 1 mM Penicillium cyclopium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.11 dextran + H2O Penicillium cyclopium the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides
-
 ?
3.2.1.11 dextran + H2O Penicillium cyclopium CICC-4022 the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Penicillium cyclopium
-
-
-
3.2.1.11 Penicillium cyclopium CICC-4022
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11 ammonium sulfate precipitation and Sepharose column chromatography Penicillium cyclopium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran Penicillium cyclopium isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides
-
 ?
3.2.1.11 dextran + H2O the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran Penicillium cyclopium CICC-4022 isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides
-
 ?
3.2.1.11 dextran T20 + H2O 90.64% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T20 + H2O 90.64% activity compared to dextran T70 Penicillium cyclopium CICC-4022 isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T2000 + H2O 97% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T2000 + H2O 97% activity compared to dextran T70 Penicillium cyclopium CICC-4022 isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T3 + H2O 65.17% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T40 + H2O 98.5% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T5 + H2O 65.54% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T500 + H2O 99.62% activity compared to dextran T70 Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 dextran T70 + H2O 100% activity Penicillium cyclopium isomaltose + isomaltotriose + D-glucose
-
 ?
3.2.1.11 additional information the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin Penicillium cyclopium ?
-
 ?
3.2.1.11 additional information the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin Penicillium cyclopium CICC-4022 ?
-
 ?
3.2.1.11 soluble starch + H2O 20.83% activity compared to dextran T70 Penicillium cyclopium ?
-
 ?
3.2.1.11 soluble starch + H2O 20.83% activity compared to dextran T70 Penicillium cyclopium CICC-4022 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.11 ? x * 66000, SDS-PAGE Penicillium cyclopium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.11 1,6-alpha-D-glucan-6-glucanohydrolase
-
 Penicillium cyclopium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 55
-
-
 Penicillium cyclopium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.11 35 60 when temperature varying from 35 to 60°C, the relative activity is always higher than 54.55% Penicillium cyclopium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.11 45 50 after 60 min 45°C and 50°C, the dextranase activity remains stable with the values being 98.09% and 62.21% of relative enzymatic activity, respectively Penicillium cyclopium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.11 208.45
-
 dextran T5 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 211.97
-
 dextran T70 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 223.53
-
 dextran T2000 at pH 5.0 and 50°C Penicillium cyclopium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 5
-
-
 Penicillium cyclopium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.11 3 6 with pH ranged from 3.0 to 6.0, the relative enzyme activity is higher than 72.67% Penicillium cyclopium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.11 2.5 7 the relative enzymatic activity of dextranase stored at pH 2.5-7.0 for 1 h and 24 h, is 89.22% and 84.58%, respectively Penicillium cyclopium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.11 76.636
-
 dextran T5 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 81.215
-
 dextran T70 at pH 5.0 and 50°C Penicillium cyclopium
3.2.1.11 91.237
-
 dextran T2000 at pH 5.0 and 50°C Penicillium cyclopium