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Literature summary extracted from

  • Morales-Luna, L.; Serrano-Posada, H.; Gonzalez-Valdez, A.; Ortega-Cuellar, D.; Vanoye-Carlo, A.; Hernandez-Ochoa, B.; Sierra-Palacios, E.; Rufino-Gonzalez, Y.; Castillo-Rodriguez, R.A.; Perez de la Cruz, V.; Moreno-Vargas, L.; Prada-Gracia, D.; Marcial-Quino, J.; Gomez-Manzo, S.
    Biochemical characterization and structural modeling of fused glucose-6-phosphate dehydrogenase-phosphogluconolactonase from Giardia lamblia (2018), Int. J. Mol. Sci., 19, 2518 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.31 expression in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.31 homology modeling of structure Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.31 guanidine hydrochloride inhibitory at 0.2 M, 40% inhibition at 0.4 M, 65% inhibition at 0.5 M Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.31 170000
-
 gel filtration Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.31 Giardia intestinalis A8BWM5 bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase, ECs 1.1.1.49 and 3.1.1.31, respectively
-
3.1.1.31 Giardia intestinalis ATCC 50803 A8BWM5 bifunctional glucose-6-phosphate 1-dehydrogenase/6-phosphogluconolactonase, ECs 1.1.1.49 and 3.1.1.31, respectively
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.31 the dimer undergoes dissociation at low concentrations, and the monomeric state is catalytically inactive. The enzyme maintains 100% of its activity when it is incubated at a concentration of 50 microg/ml Giardia intestinalis

Subunits

EC Number Subunits Comment Organism
3.1.1.31 homodimer 2 * 83000, calculated from sequence Giardia intestinalis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.31 GL50803_8682 locus name Giardia intestinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.31 37 42.5
-
 Giardia intestinalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.31 49.3
-
 50% loss of activity Giardia intestinalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.31 57
-
 melting temperature Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.31 8.8
-
-
 Giardia intestinalis

Expression

EC Number Organism Comment Expression
3.1.1.31 Giardia intestinalis enzyme is active from the log phase on and during the stationary phase additional information