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Literature summary extracted from

  • Zhu, Y.; Liu, H.; Qiao, C.; Li, L.; Jiang, Z.; Xiao, A.; Ni, H.
    Characterization of an arylsulfatase from a mutant library of Pseudoalteromonas carrageenovora arylsulfatase (2017), Int. J. Biol. Macromol., 96, 370-376 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.1 H260L mutant displays increased thermostability and increased half-life at 55°C Pseudoalteromonas carrageenovora

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 2-mercaptoethanol 1 mM, 81% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Cd2+ 1 mM, 65% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Cu2+ 1 mM, 9% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 dithiothreitol 1 mM, 69.8% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 EDTA 1 mM, 125.6% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 guanidine hydrochloride 1 mM, 69.5% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Mg2+ 1 mM, 88% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Urea 1 mM, 86% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Zn2+ 1 mM, complete loss of activity, mutant H260L Pseudoalteromonas carrageenovora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 0.66
-
 4-nitrophenyl sulfate mutant H260L, pH 7.5, 55°C Pseudoalteromonas carrageenovora
3.1.6.1 0.71
-
 4-nitrophenyl sulfate wild-type, pH 7.5, 55°C Pseudoalteromonas carrageenovora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.6.1 Ba2+ 1 mM, 110% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Ca2+ 10 mM, 137% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Co2+ 1 mM, 119% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Mn2+ 10 mM, 230% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
3.1.6.1 Na+ 1 mM, 108% of initial activity, mutant H260L Pseudoalteromonas carrageenovora

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Pseudoalteromonas carrageenovora P28607
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-nitrophenyl sulfate + H2O
-
 Pseudoalteromonas carrageenovora 4-nitrophenol + sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.1 55
-
 mutant H260L Pseudoalteromonas carrageenovora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.1 8
-
 mutant H260L Pseudoalteromonas carrageenovora

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.6.1 6 9 mutant H260L Pseudoalteromonas carrageenovora