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Literature summary extracted from

  • Xiao, Q.; Yin, Q.; Ni, H.; Cai, H.; Wu, C.; Xiao, A.
    Characterization and immobilization of arylsulfatase on modified magnetic nanoparticles for desulfation of agar (2017), Int. J. Biol. Macromol., 94, 576-584 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.6.1 synthesis immobilization of enzyme on carboxyl functioned magnetic nanoparticles using glutaraldehyde. Immobilization leads to increased tolerance to some metal ions, inhibitors and detergents. Immobilized arylsulfatase maintains 61.7% of its initial desulfuration rates after seven cycles. After the reaction of agar with immobilized arylsulfatase for 90 min at 50°C, 46% of the sulfate in the agar is removed Pseudoalteromonas carrageenovora

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 2-mercaptoethanol 1 mM, 70% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Ag+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Al3+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Cd2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Cu2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Fe2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 Fe3+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
3.1.6.1 PMSF 1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
3.1.6.1 SDS 1 mM, 5% residual activity, free enzyme, 45% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
3.1.6.1 Triton X-100 1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
3.1.6.1 Tween 80 1 mM, 10% residual activity, free enzyme, 60% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
3.1.6.1 Zn2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 0.89
-
 4-nitrophenyl sulfate pH 7.5, 50°C, immobilized enzyme Pseudoalteromonas carrageenovora

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Pseudoalteromonas carrageenovora P28607
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-nitrophenyl sulfate + H2O
-
 Pseudoalteromonas carrageenovora 4-nitrophenol + sulfate
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.1 256.9
-
 4-nitrophenyl sulfate pH 7.5, 50°C, immobilized enzyme Pseudoalteromonas carrageenovora