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Literature summary extracted from

  • Sassi, A.H.; Tounsi, H.; Trigui-Lahiani, H.; Bouzouita, R.; Romdhane, Z.B.; Gargouri, A.
    A low-temperature polygalacturonase from P. occitanis characterization and application in juice clarification (2016), Int. J. Biol. Macromol., 91, 158-164 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.15 Urea 122% activity at 5 mM Penicillium sp. 'occitanis'

Application

EC Number Application Comment Organism
3.2.1.15 food industry the enzyme is used for juice clarification of pear, banana and citrus Penicillium sp. 'occitanis'

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ba2+ complete inhibition at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 Ca2+ 64.9% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 Co2+ 0.31% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 Cu2+ 2.48% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 diethyl dicarbonate complete inhibition at 0.1% (w/v) Penicillium sp. 'occitanis'
3.2.1.15 EDTA 80% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 Fe2+ 16.45% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 K+ 87.5% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 Mn2+ complete inhibition at 1 mM Penicillium sp. 'occitanis'
3.2.1.15 additional information iodoacetamide, iodoacetate, dithiothreitol, 2-mercaptoethanol and Triton-X100 have no significant effect on enzyme activity Penicillium sp. 'occitanis'
3.2.1.15 Na+ 84.4% residual activity at 2 mM Penicillium sp. 'occitanis'
3.2.1.15 SDS 70.4% residual activity at 0.5% (w/v) Penicillium sp. 'occitanis'

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Mg2+ 132.9% activity at 1 mM Penicillium sp. 'occitanis'

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Penicillium sp. 'occitanis'
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 anion exchange column chromatography Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O
-
 Penicillium sp. 'occitanis' ?
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Penicillium sp. 'occitanis' oligogalacturonic acid + digalacturonic acid + trigalacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 42000, SDS-PAGE Penicillium sp. 'occitanis'

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-polygalacturonase
-
 Penicillium sp. 'occitanis'
3.2.1.15 PG2
-
 Penicillium sp. 'occitanis'
3.2.1.15 PGase
-
 Penicillium sp. 'occitanis'

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 35
-
-
 Penicillium sp. 'occitanis'

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 25 50 about 40% activity at 25°C, about 70% activity at 30°C, 100% activity at 35°C, about 80% activity at 40°C, about 40% activity at 50°C Penicillium sp. 'occitanis'

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 30 50 the enzyme retains about 90%, 80%, 75% and 10% activity after 7 h at 30°C, 35°C, 40°C, and 50°C, respectively Penicillium sp. 'occitanis'

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 6
-
-
 Penicillium sp. 'occitanis'

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 4 9 about 5% activity at pH 4.0, about 70% activity at pH 5.0, 100% activity at pH 6.0, about 85% activity at pH 7.0, about 50% activity at pH 8.0, about 5% activity at pH 9.0 Penicillium sp. 'occitanis'

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 4 9 the enzyme retains more than 80% activity after 24 h incubation at pH 4.0-9.0 Penicillium sp. 'occitanis'