Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kabir, S.; Rahman, M.; Tasnim, S.; Karim, M.; Khatun, N.; Hasan, I.; Amin, R.; Islam, S.; Nurujjaman, M.; Kabir, A.; Sana, N.; Ozeki, Y.; Asaduzzaman, A.
    Purification and characterization of a novel chitinase from Trichosanthes dioica seed with antifungal activity (2016), Int. J. Biol. Macromol., 84, 62-68 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.200 Ca2+ 5% loss of activity at 20 mM Trichosanthes dioica
3.2.1.200 EDTA 15% loss of activity at 20 mM Trichosanthes dioica
3.2.1.200 Urea 20% loss of activity at 8 M Trichosanthes dioica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.200 Mg2+ enzyme activity increases significantly in the presence of 20 mM Mg2+ Trichosanthes dioica
3.2.1.200 Mn2+ enzyme activity increases significantly in the presence of 20 mM Mn2+ Trichosanthes dioica

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.200 Trichosanthes dioica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.200 glycoprotein
-
 Trichosanthes dioica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.200 QA-cellulose column chromatography Trichosanthes dioica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.200 seed
-
 Trichosanthes dioica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.200 colloidal chitin + H2O
-
 Trichosanthes dioica N-acetyl D-glucosamine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.200 ? x * 39000, SDS-PAGE Trichosanthes dioica

Synonyms

EC Number Synonyms Comment Organism
3.2.1.200 TdsC
-
 Trichosanthes dioica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.200 50 60
-
 Trichosanthes dioica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.200 30 80 more than 90% activity between 30 and 80°C Trichosanthes dioica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.200 90
-
 the enzyme retains 90% activity after 30 min at 90°C Trichosanthes dioica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.200 5 8
-
 Trichosanthes dioica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.200 4 10 about 50% activity at pH 4.0, 100% activity at pH 5.0-8.0, about 90% activity at pH 9.0-10.0 Trichosanthes dioica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.200 5 10 the enzyme remains stable after 30 min at pH 5.0-10.0, 80% activity is left after 30 min at pH 4.0 and 11.0 Trichosanthes dioica

General Information

EC Number General Information Comment Organism
3.2.1.200 physiological function the enzyme shows antifungal activity against Aspergillus niger and Trichoderma sp. Trichosanthes dioica