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Literature summary extracted from

  • Zhang, J.; Cao, H.; Li, S.; Zhao, Y.; Wang, W.; Xu, Q.; Du, Y.; Yin, H.
    Characterization of a new family 75 chitosanase from Aspergillus sp. W-2 (2015), Int. J. Biol. Macromol., 81, 362-369 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 synthesis the enzyme can be a competitive candidate for chitosan oligosaccharide-manufacturing industry Aspergillus sp. W-2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 the mature CsnW2 protein is fused to His-tag at C-terminus and expressed in Pichia pastoris X-33 Aspergillus sp. W-2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Cu2+ 1 mM, 90% loss of activity Aspergillus sp. W-2
3.2.1.132 Fe2+ 1 mM, 39% loss of activity Aspergillus sp. W-2
3.2.1.132 Ge2+ 1 mM, 83% loss of activity Aspergillus sp. W-2
3.2.1.132 Ni2+ 1 mM, 84% loss of activity Aspergillus sp. W-2
3.2.1.132 Zn2+ 1 mM, 75% loss of activity Aspergillus sp. W-2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 chitosan Km-value: 7.1 mg/ml, pH 6.0, 55°C Aspergillus sp. W-2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ 1 mM, 1.1fold activation Aspergillus sp. W-2
3.2.1.132 Mg2+ 1 mM, 1.1fold activation Aspergillus sp. W-2
3.2.1.132 Mn2+ 1 mM, 1.2fold activation Aspergillus sp. W-2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 25148
-
 calculated from sequence Aspergillus sp. W-2

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Aspergillus sp. W-2 A0A0B5L773
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Aspergillus sp. W-2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + H2O (GlcN)6 is mainly hydrolyzed into two molecules of (GlcN)3, accompanied by a very small proportion of (GlcN)2 and (GlcN)4 Aspergillus sp. W-2 beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
-
 ?
3.2.1.132 chitosan + H2O the enzyme prefers to hydrolyze chitosan with high degree of deacetylation. The main products of chitiosan (92% deacetylation) are chitosan oligosaccharides with degree of polymerization (DP) ranging from 2 to 6. No activity with colloidal chitin, carboxymethylcellulose, microcrystalline cellulose, glucosan Aspergillus sp. W-2 chitosan oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 csnw2
-
 Aspergillus sp. W-2
3.2.1.132 endo-acting chitinase
-
 Aspergillus sp. W-2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 55
-
-
 Aspergillus sp. W-2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 75
-
 0.5 h, 16% loss of activity. 1 h, 22% loss of activity Aspergillus sp. W-2

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 0.47
-
 chitosan pH 6.0, 55°C Aspergillus sp. W-2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6
-
-
 Aspergillus sp. W-2

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 3 10 1 h, the enzyme retains over 79% of its activity Aspergillus sp. W-2