EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expression in Escherichia coli BL21 (DE3) | Ruminococcus bromii |
3.2.1.1 | gene RB5AMG_01539, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RP | Ruminococcus bromii |
3.2.1.135 | expression in Escherichia coli BL21 (DE3) | Ruminococcus bromii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
additional information | kinetics with different substrates, overview | Ruminococcus bromii | |
3.2.1.1 | additional information | - |
pullulan | Km-value: 15.4 mg/ml, 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | additional information | - |
starch | Km-value: 16.0 mg/ml, 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | additional information | - |
amylose | Km-value: 6.17 mg/ml, 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | additional information | - |
amylopectin | Km-value: 8.95 mg/ml, 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.135 | additional information | - |
pullulan | Km-value: 15.4 mg/ml, 45°C, pH 5.0 | Ruminococcus bromii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.1 | extracellular | - |
Ruminococcus bromii | - |
- |
3.2.1.135 | extracellular | - |
Ruminococcus bromii | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.135 | pullulan + H2O | Ruminococcus bromii | the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine | panose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Ruminococcus bromii | - |
- |
- |
3.2.1.1 | Ruminococcus bromii | A0A2N0UQI0 | - |
- |
3.2.1.1 | Ruminococcus bromii ATCC 27255 | - |
- |
- |
3.2.1.1 | Ruminococcus bromii ATCC 27255 | A0A2N0UQI0 | - |
- |
3.2.1.135 | Ruminococcus bromii | - |
- |
- |
3.2.1.135 | Ruminococcus bromii ATCC 27255 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | - |
Ruminococcus bromii |
3.2.1.1 | recombinant His6-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RP by nickel affinity chromatography and ultrafiltration | Ruminococcus bromii |
3.2.1.135 | - |
Ruminococcus bromii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 46.1 | - |
purified recombinant His-tagged enzyme, pH 5.0, 45°C, substrate amylopectin | Ruminococcus bromii |
3.2.1.1 | 72.5 | - |
45°C, pH 5.0 | Ruminococcus bromii |
3.2.1.1 | 72.5 | - |
purified recombinant His-tagged enzyme, pH 5.0, 45°C, substrate pullulan | Ruminococcus bromii |
3.2.1.1 | 254 | - |
purified recombinant His-tagged enzyme, pH 5.0, 45°C, substrate soluble starch | Ruminococcus bromii |
3.2.1.1 | 384 | - |
purified recombinant His-tagged enzyme, pH 5.0, 45°C, substrate amylose | Ruminococcus bromii |
3.2.1.135 | 72.5 | - |
45°C, pH 5.0 | Ruminococcus bromii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | at 12% of the activity as compared to amylose | Ruminococcus bromii | ? | - |
? | |
3.2.1.1 | amylopectin + H2O | at 12% of the activity as compared to amylose | Ruminococcus bromii ATCC 27255 | ? | - |
? | |
3.2.1.1 | amylopectin + H2O | 0.5% substrate solution, 12% activity compared to amylose | Ruminococcus bromii | maltose + D-glucose | - |
? | |
3.2.1.1 | amylose + H2O | - |
Ruminococcus bromii | ? | - |
? | |
3.2.1.1 | amylose + H2O | - |
Ruminococcus bromii ATCC 27255 | ? | - |
? | |
3.2.1.1 | amylose + H2O | 0.5% substrate solution, best substrate | Ruminococcus bromii | maltose + D-glucose | - |
? | |
3.2.1.1 | amylose + H2O | 0.5% substrate solution, best substrate | Ruminococcus bromii ATCC 27255 | maltose + D-glucose | - |
? | |
3.2.1.1 | gamma-cyclodextrin + H2O | at 3.9% of the activity as compared to amylose | Ruminococcus bromii | ? | - |
? | |
3.2.1.1 | gamma-cyclodextrin + H2O | at 3.9% of the activity as compared to amylose | Ruminococcus bromii ATCC 27255 | ? | - |
? | |
3.2.1.1 | maltopentaose + H2O | - |
Ruminococcus bromii | maltose + ? | - |
? | |
3.2.1.1 | maltopentaose + H2O | - |
Ruminococcus bromii | maltotriose + maltose + D-glucose | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
Ruminococcus bromii | 2 maltose | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
Ruminococcus bromii ATCC 27255 | 2 maltose | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
Ruminococcus bromii | maltose + ? | - |
? | |
3.2.1.1 | maltotriose + H2O | - |
Ruminococcus bromii | maltose + D-glucose | - |
? | |
3.2.1.1 | maltotriose + H2O | - |
Ruminococcus bromii ATCC 27255 | maltose + D-glucose | - |
? | |
3.2.1.1 | maltotriose + H2O | - |
Ruminococcus bromii | maltose + ? | - |
? | |
3.2.1.1 | additional information | alpha-cyclodextrin, beta-cyclodextrin, gamma-cyclodextrin, and malto-beta-cyclodextrin are poor substrates. Thin layer chromatography and high performance anion exchange chromatography analysis of hydrolysis products from maltodextrins (G2-G5) by recombinant enzyme Rbamy5, overview. Maltose is not hydrolyzed | Ruminococcus bromii | ? | - |
? | |
3.2.1.1 | additional information | alpha-cyclodextrin, beta-cyclodextrin, gamma-cyclodextrin, and malto-beta-cyclodextrin are poor substrates. Thin layer chromatography and high performance anion exchange chromatography analysis of hydrolysis products from maltodextrins (G2-G5) by recombinant enzyme Rbamy5, overview. Maltose is not hydrolyzed | Ruminococcus bromii ATCC 27255 | ? | - |
? | |
3.2.1.1 | pullulan + H2O | 0.5% substrate solution, 18.9% activity compared to amylose | Ruminococcus bromii | panose + ? | - |
? | |
3.2.1.1 | pullulan + H2O | at 72.5% of the activity as compared to amylose. The enzyme is an alpha-amylase with neopullulanase-like activity | Ruminococcus bromii | panose + ? | - |
? | |
3.2.1.1 | pullulan + H2O | at 72.5% of the activity as compared to amylose. The enzyme is an alpha-amylase with neopullulanase-like activity | Ruminococcus bromii ATCC 27255 | panose + ? | - |
? | |
3.2.1.1 | pullulan + H2O | 0.5% substrate solution, 18.9% activity compared to amylose | Ruminococcus bromii ATCC 27255 | panose + ? | - |
? | |
3.2.1.1 | soluble starch + H2O | 0.5% substrate solution, 66.2% activity compared to amylose | Ruminococcus bromii | maltose + D-glucose | - |
? | |
3.2.1.1 | starch + H2O | at 66.2% of the activity as compared to amylose | Ruminococcus bromii | ? | - |
? | |
3.2.1.1 | starch + H2O | at 66.2% of the activity as compared to amylose | Ruminococcus bromii ATCC 27255 | ? | - |
? | |
3.2.1.135 | pullulan + H2O | the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine | Ruminococcus bromii | panose + ? | - |
? | |
3.2.1.135 | pullulan + H2O | the enzyme is an alpha-amylase with neopullulanase-like activity | Ruminococcus bromii | panose + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | ? | x * 60000, recombinant His6-tagged enzyme, SDS-PAGE, x * 58500, about, sequence calculation | Ruminococcus bromii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | amy5 | - |
Ruminococcus bromii |
3.2.1.1 | RB5AMG_01539 | - |
Ruminococcus bromii |
3.2.1.1 | Rbamy5 | - |
Ruminococcus bromii |
3.2.1.1 | Ruminococcus bromii intermediary alpha-amylase 5 | - |
Ruminococcus bromii |
3.2.1.135 | Rbamy5 | - |
Ruminococcus bromii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 45 | - |
recombinant enzyme | Ruminococcus bromii |
3.2.1.1 | 45 | - |
substrate: soluble starch | Ruminococcus bromii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 40% of maximal activity, substrate: soluble starch | Ruminococcus bromii |
3.2.1.1 | 30 | 50 | 40% of maximal activity at 30°C and 50°C, profile overview | Ruminococcus bromii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 35 | - |
140 min, no loss of activity | Ruminococcus bromii |
3.2.1.1 | 35 | - |
purified recombinant enzyme, completely stable for 140 min | Ruminococcus bromii |
3.2.1.1 | 40 | - |
120 min, about 45% loss of activity | Ruminococcus bromii |
3.2.1.1 | 40 | - |
purified recombinant enzyme, loss of 50% activity after 140 min | Ruminococcus bromii |
3.2.1.1 | 45 | - |
20 min, about 70% loss of activity | Ruminococcus bromii |
3.2.1.1 | 45 | - |
purified recombinant enzyme, inactivation within about 27 min | Ruminococcus bromii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 137 | - |
amylopectin | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 137 | - |
amylopectin | recombinant His-tagged enzyme, pH 5.0, 45°C | Ruminococcus bromii | |
3.2.1.1 | 297 | - |
pullulan | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 297 | - |
pullulan | recombinant His-tagged enzyme, pH 5.0, 45°C | Ruminococcus bromii | |
3.2.1.1 | 1014 | - |
amylose | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 1014 | - |
amylose | recombinant His-tagged enzyme, pH 5.0, 45°C | Ruminococcus bromii | |
3.2.1.1 | 1055 | - |
starch | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 1055 | - |
soluble starch | recombinant His-tagged enzyme, pH 5.0, 45°C | Ruminococcus bromii | |
3.2.1.135 | 297 | - |
pullulan | 45°C, pH 5.0 | Ruminococcus bromii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | - |
recombinant enzyme | Ruminococcus bromii |
3.2.1.1 | 5 | - |
substrate: soluble starch | Ruminococcus bromii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 4 | 9 | 30% of maxial activity at pH 4.0 and pH 9.0, profile overview | Ruminococcus bromii |
3.2.1.1 | 4 | 9 | pH 4.0: about 30% of maximal activity, pH 9.0: about 30% of maximal activity, substrate: soluble starch | Ruminococcus bromii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.1 | evolution | the enzyme belongs to the glycosyl hydrolase family 13, GH13 | Ruminococcus bromii |
3.2.1.1 | physiological function | the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine | Ruminococcus bromii |
3.2.1.135 | physiological function | the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine | Ruminococcus bromii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 15.4 | - |
amylopectin | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 19.3 | - |
pullulan | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 65.8 | - |
starch | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.1 | 164 | - |
amylose | 45°C, pH 5.0 | Ruminococcus bromii | |
3.2.1.135 | 19.3 | - |
pullulan | 45°C, pH 5.0 | Ruminococcus bromii |