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Literature summary extracted from

  • Rai, S.; Yadav, S.; Rai, R.; Chatterjee, A.; Singh, S.; Rai, L.C.
    Molecular and biochemical characterization of All0580 as a methylglyoxal detoxifying glyoxalase II of Anabaena sp. PCC7120 that confers abiotic stress tolerance in E. coli (2019), Int. J. Biol. Macromol., 124, 981-993 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 expressed in Escherichia coli BL21 DELTAgloB cells Nostoc sp. PCC 7120 = FACHB-418

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 GSH
-
 Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.2982
-
 S-D-lactoylglutathione at pH 7.5 and 25°C Nostoc sp. PCC 7120 = FACHB-418

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ the enzyme contains a binuclear Zn/Fe centre Nostoc sp. PCC 7120 = FACHB-418
3.1.2.6 Zn2+ the enzyme contains a binuclear Zn/Fe centre Nostoc sp. PCC 7120 = FACHB-418

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Nostoc sp. PCC 7120 = FACHB-418 Q8YZ99
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6 Ni-NTA column chromatography Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-D-lactoylglutathione + H2O
-
 Nostoc sp. PCC 7120 = FACHB-418 D-lactate + glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.6 ? x * 28700, SDS-PAGE Nostoc sp. PCC 7120 = FACHB-418

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 All0580
-
 Nostoc sp. PCC 7120 = FACHB-418
3.1.2.6 GLYII
-
 Nostoc sp. PCC 7120 = FACHB-418
3.1.2.6 glyoxalase II
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.5
-
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.6 6.5 8 enhanced enzyme activity in the pH range of 6.5-8.0 Nostoc sp. PCC 7120 = FACHB-418

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.2.6 2.5
-
 at pH 7.5 and 25°C Nostoc sp. PCC 7120 = FACHB-418 GSH

General Information

EC Number General Information Comment Organism
3.1.2.6 physiological function the enzyme confers tolerance to abiotic stresses (salinity, drought, arsenic, heat, and UV-B stresses) Nostoc sp. PCC 7120 = FACHB-418

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.6 3000
-
 S-D-lactoylglutathione at pH 7.5 and 25°C Nostoc sp. PCC 7120 = FACHB-418