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Literature summary extracted from

  • Zhao, J.; Guo, C.; Zhang, L.; Tian, C.
    Biochemical and functional characterization of a novel thermoacidophilic, heat and halo-ionic liquids tolerant endo-beta-1,4-glucanase from saline-alkaline lake soil microbial metagenomic DNA (2018), Int. J. Biol. Macromol., 118, 1035-1044 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 2-mercaptoethanol
-
 uncultured bacterium
3.2.1.4 DTT
-
 uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene nmGH45, cloned from saline-alkaline lake soil microbial metagenomic DNA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Pichia pastoris strain GS115, co-expression of molecular chaperone BIP enhances enzyme protein secretion by 20%, but chaperones PDI and HAC1 have limited influences uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 1-butyl-3-methylimidazolium chloride 92.8% and 43.8% activity remaining in 10% and 20% 1-butyl-3-methylimidazolium chloride uncultured bacterium
3.2.1.4 Ca2+ slight inhibition uncultured bacterium
3.2.1.4 EDTA slight inhibition uncultured bacterium
3.2.1.4 Pb2+ slight inhibition uncultured bacterium
3.2.1.4 SDS strong inhibition uncultured bacterium
3.2.1.4 Triton X-100 slight inhibition uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Co2+ activates slightly uncultured bacterium
3.2.1.4 Cu2+ activates uncultured bacterium
3.2.1.4 Mn2+ activates uncultured bacterium
3.2.1.4 additional information no or poor effects by Li+, K+, Na+, Mg2+, and Fe3+ uncultured bacterium
3.2.1.4 NaCl enzyme NMgh45 retains 88% activity in 4 M NaCl after 24 h uncultured bacterium
3.2.1.4 Ni2+ activates slightly uncultured bacterium
3.2.1.4 Zn2+ activates uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium A0A286Q5D2 isolated from saline-alkaline lake soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant enzyme from Pichia pastoris strain GS115 uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 8.13
-
 purified recombinant enzyme, pH 5.0, 60°C, substrate laminarin uncultured bacterium
3.2.1.4 10.9
-
 purified recombinant enzyme, pH 5.0, 60°C, substrate Avicel PH-101 uncultured bacterium
3.2.1.4 65.53
-
 purified recombinant enzyme, pH 5.0, 60°C, substrate carboxymethyl cellulose uncultured bacterium
3.2.1.4 92.29
-
 purified recombinant enzyme, pH 5.0, 60°C, substrate barley beta-glucan uncultured bacterium
3.2.1.4 134.22
-
 purified recombinant enzyme, pH 5.0, 60°C, substrate lichenin uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 Avicel PH-101 + H2O low activity uncultured bacterium ?
-
 ?
3.2.1.4 barley beta-glucan + H2O moderate activity uncultured bacterium ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O moderate activity uncultured bacterium ?
-
 ?
3.2.1.4 laminarin + H2O low activity uncultured bacterium ?
-
 ?
3.2.1.4 lichenin + H2O best substrate uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 22500, about, sequence calculation, x * 37000, recombinant enzyme, SDS-PAGE uncultured bacterium
3.2.1.4 More modeled tertiary structure of NMgh45, overview uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endo-beta-1,4-glucanase
-
 uncultured bacterium
3.2.1.4 nmGH45
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60 70
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 40 80 over 60% of maximal activity within this range, profile overview uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60 70 recombinant enzyme NMgh45, completely stable for 2 h uncultured bacterium
3.2.1.4 80
-
 recombinant enzyme NMgh45, 90% activity remains after 2 h uncultured bacterium
3.2.1.4 90
-
 recombinant enzyme NMgh45, 68% activity remains after 1 h, 40% after 2 h uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.5
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3 9 and below, over 50% of maximal activity within this range, profile overview uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 11 recombinant enzyme NMgh45, over 80% activity remains after 24 h uncultured bacterium

General Information

EC Number General Information Comment Organism
3.2.1.4 evolution the enzyme belongs to the glycosyl hydrolase family 45, GH45 uncultured bacterium
3.2.1.4 additional information compared with other non-halotolerant counterparts, enzyme NMgh45 is enriched with acidic amino acids on the protein surface, and the conformation of NMgh45 is stable at high salinity condition, three-dimensional structure, molecular modeling and dynamic simulations using the structures with PDB IDs 1L8F, 2ENG, and 5GLX_A as the templates, overview uncultured bacterium