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Literature summary extracted from

  • Han, C.; Li, W.; Hua, C.; Sun, F.; Bi, P.; Wang, Q.
    Enhancement of catalytic activity and thermostability of a thermostable cellobiohydrolase from Chaetomium thermophilum by site-directed mutagenesis (2018), Int. J. Biol. Macromol., 116, 691-697 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Pichia pastoris GS115 cells Thermochaetoides thermophila

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 S131W the mutant with reduced activity exhibits effectively enhanced heat resistance to elevated temperatures. The half-life of this mutant enzyme is increased 1.42 and 2.40fold at 80°C and 90°C, respectively, compared to the wild type Thermochaetoides thermophila
3.2.1.91 W221R the mutant shows significantly declined activity compared to the wild type enzyme Thermochaetoides thermophila
3.2.1.91 W315R the mutant shows significantly declined activity compared to the wild type enzyme Thermochaetoides thermophila
3.2.1.91 Y119F the mutation increases the catalytic activity 1.82, 1.65 and 1.43fold against beta-D-glucan, phosphoric acid swollen cellulose and carboxymethyl cellulose, respectively, compared to the wild type enzyme Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Thermochaetoides thermophila G0S9B2
-
-
3.2.1.91 Thermochaetoides thermophila DSM 1495 G0S9B2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 HisTrap column chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 beta-D-glucan + H2O highest activity Thermochaetoides thermophila ?
-
 ?
3.2.1.91 beta-D-glucan + H2O highest activity Thermochaetoides thermophila DSM 1495 ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O lowest activity Thermochaetoides thermophila ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O lowest activity Thermochaetoides thermophila DSM 1495 ?
-
 ?
3.2.1.91 phosphoric acid swollen cellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
3.2.1.91 phosphoric acid swollen cellulose + H2O
-
 Thermochaetoides thermophila DSM 1495 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 45000, SDS-PAGE Thermochaetoides thermophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 Cel6
-
 Thermochaetoides thermophila
3.2.1.91 cellobiohydrolase
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 70
-
-
 Thermochaetoides thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.91 40 90 more than 50% activity between 40 and 90°C Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 40 90 after 1 h at 40, 50, 60, 70, 80, and 90°C, the wild type enzyme retains about 100%, 99%, 93%, 70%, 47%, and 21% activity, respectively. The half-life at 90°C is 25 min Thermochaetoides thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 0.00022
-
 Beta-D-glucan mutant enzyme W315R, at pH 5.0 and 70°C Thermochaetoides thermophila
3.2.1.91 0.00047
-
 Beta-D-glucan mutant enzyme W221R, at pH 5.0 and 70°C Thermochaetoides thermophila
3.2.1.91 0.00054
-
 Beta-D-glucan wild type enzyme, at pH 5.0 and 70°C Thermochaetoides thermophila
3.2.1.91 0.00073
-
 Beta-D-glucan mutant enzyme S131W, at pH 5.0 and 70°C Thermochaetoides thermophila
3.2.1.91 0.00118
-
 Beta-D-glucan mutant enzyme Y119F, at pH 5.0 and 70°C Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
-
 Thermochaetoides thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.91 4 10 more than 50% activity between pH 4.0 and 10.0 Thermochaetoides thermophila