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Literature summary extracted from

  • Busswinkel, F.; Goni, O.; Cord-Landwehr, S.; OConnell, S.; Moerschbacher, B.M.
    Endochitinase 1 (Tv-ECH1) from Trichoderma virens has high subsite specificities for acetylated units when acting on chitosans (2018), Int. J. Biol. Macromol., 114, 453-461 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.14 biotechnology the enzyme is used for the biotechnological production of specific chitosan oligomers and for the characterization of chitosan polymers via enzymatic fingerprinting Trichoderma virens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expressed in Pichia pastoris PichiaPink Strain 1 Trichoderma virens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Trichoderma virens Q8TGW8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 Ni-NTA column chromatography Trichoderma virens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl chitotrioside + H2O
-
 Trichoderma virens ?
-
 ?
3.2.1.14 beta-D-N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Trichoderma virens beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Trichoderma virens beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Trichoderma virens 2 beta-D-N,N'-diacetylchitobiose
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Trichoderma virens ?
-
 ?
3.2.1.14 additional information the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose, beta-D-N,N',N''-triacetylchitotriose, alpha-chitin and beta-chitin Trichoderma virens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 45000, SDS-PAGE Trichoderma virens
3.2.1.14 ? x * 45300, calculated from amino acid sequence Trichoderma virens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ECH1
-
 Trichoderma virens
3.2.1.14 endochitinase 1
-
 Trichoderma virens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37
-
-
 Trichoderma virens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 20 50 more than 50% activity between 20 and 50°C Trichoderma virens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 37
-
 the enzyme remains stable for at least 50 h at pH 4.5 and 37°C Trichoderma virens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 8.08
-
 colloidal chitin at pH 4.5 and 37°C Trichoderma virens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4.5
-
-
 Trichoderma virens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4.5 6 more than 90% activity between pH 4.5 and 6.0 Trichoderma virens