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Literature summary extracted from

  • Habrylo, O.; Evangelista, D.E.; Castilho, P.V.; Pelloux, J.; Henrique-Silva, F.
    The pectinases from Sphenophorus levis Potential for biotechnological applications (2018), Int. J. Biol. Macromol., 112, 499-508 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.11 food industry the enzyme is suitable for both acidic and alkaline processing, such as coffee and tea fermentation Sphenophorus levis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 sequence comparisons, the Sl-pectinase ORF, excluding the coding sequence for signal peptide, is cloned in frame with His-tag into pPICZalphaA and pPICZalphaB expression vectors, recombinant overexpression in Pichia pastoris strain KM71H Sphenophorus levis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 (-)-epigallocatechin gallate exhibits 97.8% inhibition at 1 mg/ml and 47.0% at 0.1 mg/ml Sphenophorus levis
3.1.1.11 additional information no inhibition by PME inhibitor from kiwi fruits Sphenophorus levis
3.1.1.11 NaCl NaCl causes loss of 40% and 90% of enzyme activity at 0.1 M and 0.5 M, respectively Sphenophorus levis
3.1.1.11 polyphenon 60 PP60, exhibits 94.8% inhibition at 1 mg/ml and 26.8% at 0.1 mg/ml Sphenophorus levis
3.1.1.11 Tannic acid exhibits 99.5% inhibition at 1 mg/ml and 55.2% at 0.1 mg/ml Sphenophorus levis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.11 extracellular
-
 Sphenophorus levis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + n H2O Sphenophorus levis
-
 n methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Sphenophorus levis V9Q624
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.11 glycoprotein enzyme PME contains one N-glycosylation site, deglycosylation of Sl-pectinases is performed using endoglycosidase H (Endo H), peptide-N-glycosidase F (PNGase F) and O-glycosidase at 37°C Sphenophorus levis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 recombinant His-tagged enzyme from pastoris strain KM71H by nickel affinity chromatography Sphenophorus levis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 additional information the enzyme is active on pectin substrates with degree of methylesterification (DM): sugar beet pectin (DM 42%) apple pectin (DM 70-75%), and citrus pectin (DM 20-34%, 55-70%, and over 85%), but not on polygalacturonic acid Sphenophorus levis ?
-
 ?
3.1.1.11 pectin + n H2O
-
 Sphenophorus levis n methanol + pectate
-
 ?
3.1.1.11 pectin + n H2O enzyme Sl-PME shows 100% of relative activity for 85% DM pectin, 51.1% for 55-70% DM pectin, 22.4% for 42% DM pectin and only 6.6% for 20-34% DM pectin Sphenophorus levis n methanol + pectate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.11 ? x * 50000, about, recombinant His-tagged enzyme, SDS-PAGE Sphenophorus levis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Sphenophorus levis
3.1.1.11 Sl-PME
-
 Sphenophorus levis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 40
-
 recombinant enzyme Sphenophorus levis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.11 5 55 over 50% of maximal activity at 20-50°C, activity range, profile overview Sphenophorus levis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 5 45 enzyme Sl-PME remains stable up to 35°C, activity decreases by 25% at 40°C and is completely abolished at 45°C Sphenophorus levis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 7
-
 recombinant enzyme Sphenophorus levis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 6 9 inactive at pH 5.0 and above pH 10.0, over 90% of maximal activity at pH 6.0-8.0 Sphenophorus levis

General Information

EC Number General Information Comment Organism
3.1.1.11 physiological function plant and bacterial pectin methylesterases (PMEs) perform the catalysis with a processive catalytic mechanism, unlike fungal PME whose activity leads to a random repartition of non-esterified carboxyl groups Sphenophorus levis