Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Han, Y.; Yu, R.; Gao, P.; Lu, X.; Yu, W.
    The hydrogen-bond network around Glu160 contributes to the structural stability of chitosanase CsnA from Renibacterium sp. QD1 (2018), Int. J. Biol. Macromol., 109, 880-887 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 industry the enzyme has a great potential for industrial applications due to its high yield and broad pH stability Renibacterium sp. QD1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132
-
 Renibacterium sp. QD1

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 E160A mutant enzyme with decreased thermal stability and reduced specific activity Renibacterium sp. QD1
3.2.1.132 E160Q mutant enzyme with decreased thermal stability and reduced specific activity Renibacterium sp. QD1
3.2.1.132 K163A mutant enzyme with decreased thermal stability and reduced specific activity Renibacterium sp. QD1
3.2.1.132 T114A mutant enzyme with decreased thermal stability and slightly reduced specific activity Renibacterium sp. QD1

General Stability

EC Number General Stability Organism
3.2.1.132 the hydrogen-bond network around Glu160 plays important roles in stabilization of the enzyme Renibacterium sp. QD1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 26000
-
 SDS-PAGE Renibacterium sp. QD1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Renibacterium sp. QD1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Renibacterium sp. QD1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 250.87
-
 pH 5.6, 55°C, mutant enzyme K163A Renibacterium sp. QD1
3.2.1.132 261.73
-
 pH 5.6, 55°C, mutant enzyme E160A Renibacterium sp. QD1
3.2.1.132 283.93
-
 pH 5.6, 55°C, mutant enzyme E160Q Renibacterium sp. QD1
3.2.1.132 328.05
-
 pH 5.6, 55°C, mutant enzyme T114A Renibacterium sp. QD1
3.2.1.132 335.07
-
 pH 5.6, 55°C, wild-type enzyme Renibacterium sp. QD1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Renibacterium sp. QD1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 26000, SDS-PAGE Renibacterium sp. QD1

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 chitosanase CsnA
-
 Renibacterium sp. QD1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 55
-
 assay at Renibacterium sp. QD1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 35.55
-
 Tm-value of mutant enzyme E160A Renibacterium sp. QD1
3.2.1.132 36.02
-
 Tm-value of mutant enzyme E160Q Renibacterium sp. QD1
3.2.1.132 37.36
-
 Tm-value of mutant enzyme T114A Renibacterium sp. QD1
3.2.1.132 38.86
-
 Tm-value of mutant enzyme K163A Renibacterium sp. QD1
3.2.1.132 40.36
-
 Tm-value of wild-type enzyme Renibacterium sp. QD1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.6
-
 assay at Renibacterium sp. QD1