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Literature summary extracted from

  • Shehata, A.; Abd El Aty, A.; Darwish, D.; Abdel Wahab, W.; Mostafa, F.
    Purification, physicochemical and thermodynamic studies of antifungal chitinase with production of bioactive chitosan-oligosaccharide from newly isolated Aspergillus griseoaurantiacus KX010988 (2018), Int. J. Biol. Macromol., 107, 990-999 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.200 Ba2+ 63.52% residual activity at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Cu2+ complete inhibition at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Fe2+ complete inhibition at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 K+ 10% residual activity at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Mg2+ 95% residual activity at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Na+ 15% residual activity at 1 mM Aspergillus griseoaurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.200 Ca2+ 120% activity at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Mn2+ 150% activity at 1 mM Aspergillus griseoaurantiacus
3.2.1.200 Zn2+ 141.8% activity at 1 mM Aspergillus griseoaurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.200 Aspergillus griseoaurantiacus
-
-
-
3.2.1.200 Aspergillus griseoaurantiacus KX010988
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.200 7.47
-
 culture filtrate, at pH 4.5 and 40°C Aspergillus griseoaurantiacus
3.2.1.200 93.75
-
 after 12.55fold purification, at pH 4.5 and 40°C Aspergillus griseoaurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.200 bean peel + H2O
-
 Aspergillus griseoaurantiacus ?
-
 ?
3.2.1.200 bean peel + H2O
-
 Aspergillus griseoaurantiacus KX010988 ?
-
 ?
3.2.1.200 colloidal chitin + H2O 100% activity Aspergillus griseoaurantiacus ?
-
 ?
3.2.1.200 colloidal chitin + H2O 100% activity Aspergillus griseoaurantiacus KX010988 ?
-
 ?
3.2.1.200 high molecular weight chitin + H2O 90% activity compared to colloidal chitin Aspergillus griseoaurantiacus ?
-
 ?
3.2.1.200 high molecular weight chitin + H2O 90% activity compared to colloidal chitin Aspergillus griseoaurantiacus KX010988 ?
-
 ?
3.2.1.200 moderate molecular weight chitin + H2O 40% activity compared to colloidal chitin Aspergillus griseoaurantiacus ?
-
 ?
3.2.1.200 moderate molecular weight chitin + H2O 40% activity compared to colloidal chitin Aspergillus griseoaurantiacus KX010988 ?
-
 ?
3.2.1.200 additional information no activity with low molecular weight chitin. The enzyme is not active on rice bran, rice straw, corn stalks, corn leaves and bagasse Aspergillus griseoaurantiacus ?
-
 ?
3.2.1.200 additional information no activity with low molecular weight chitin. The enzyme is not active on rice bran, rice straw, corn stalks, corn leaves and bagasse Aspergillus griseoaurantiacus KX010988 ?
-
 ?
3.2.1.200 potato shell + H2O
-
 Aspergillus griseoaurantiacus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.200 ? x * 130000, SDS-PAGE Aspergillus griseoaurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.200 40
-
-
 Aspergillus griseoaurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.200 40 60 the enzyme is stable at 40°C for 15 min. The enzyme loses 40% of its activity when heated at 60°C for 60 min Aspergillus griseoaurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.200 4.5
-
-
 Aspergillus griseoaurantiacus