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Literature summary extracted from

  • Mahmoud, A.E.; Fathy, S.A.; Rashad, M.M.; Ezz, M.K.; Mohammed, A.T.
    Purification and characterization of a novel tannase produced by Kluyveromyces marxianus using olive pomace as solid support, and its promising role in gallic acid production (2018), Int. J. Biol. Macromol., 107, 2342-2350 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 Ba2+ 27% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Co2+ 32% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Hg2+ 57% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 K+ 58% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Mg2+ 10% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Mn2+ 60% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Na+ 30% inhibition at 1 mM Kluyveromyces marxianus
3.1.1.20 Zn2+ 22% inhibition at 1 mM Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.77
-
 Tannic acid pH pH 5.5, 30°C Kluyveromyces marxianus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ 12% activation at 1 mM Kluyveromyces marxianus
3.1.1.20 Cd2+ 42.2% activation at 1 mM Kluyveromyces marxianus
3.1.1.20 Cu2+ 35.8% activation at 1 mM Kluyveromyces marxianus
3.1.1.20 Fe3+ 28.5% activation at 1 mM Kluyveromyces marxianus
3.1.1.20 Li+ 14% activation at 1 mM Kluyveromyces marxianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 66620
-
 gel filtration Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Kluyveromyces marxianus
-
 gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O Kluyveromyces marxianus NRRL Y-8281
-
 gallic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Kluyveromyces marxianus
-
-
-
3.1.1.20 Kluyveromyces marxianus NRRL Y-8281
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 native enzyme 24.21fold by ammonium sulfate fractionation and gel filtration Kluyveromyces marxianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.20 additional information culture growth on olive pomace as solid support in solid state fermentation Kluyveromyces marxianus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 1026.12
-
 purified native enzyme, pH 5.5, 30°C Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 epigallocatechin gallate + H2O
-
 Kluyveromyces marxianus gallic acid + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 gallic acid + epigallocatechin
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Kluyveromyces marxianus methanol + gallic acid
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 methanol + gallic acid
-
 ?
3.1.1.20 additional information the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) Kluyveromyces marxianus ?
-
 ?
3.1.1.20 additional information the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) Kluyveromyces marxianus NRRL Y-8281 ?
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Kluyveromyces marxianus propanol + gallic acid
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 propanol + gallic acid
-
 ?
3.1.1.20 tannic acid + H2O
-
 Kluyveromyces marxianus gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Kluyveromyces marxianus NRRL Y-8281 gallic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 monomer 1 * 65000, SDS-PAGE Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 35
-
-
 Kluyveromyces marxianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 25 60 activity profile overview Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 30 70 purified native enzyme, thermostable within the temperature range 30-70°C, 30 min Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 4.5
-
 optimum 1 Kluyveromyces marxianus
3.1.1.20 8.5
-
 optimum 2 Kluyveromyces marxianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3 9 activity profile overview Kluyveromyces marxianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 4 5.5 purified native enzyme, stable in the pH range of pH 4.0-5.5 for 30 min Kluyveromyces marxianus