BRENDA - Enzyme Database

Molecular characterization and bioinformatics studies of a lipase from Bacillus thermoamylovorans BHK67

Sharma, A.; Meena, K.; Kanwar, S.; Int. J. Biol. Macromol. 107, 2131-2140 (2018)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
acetonitrile
-
Bacillus thermoamylovorans
3.1.1.5
DMSO
-
Bacillus thermoamylovorans
3.1.1.5
ethanol
-
Bacillus thermoamylovorans
3.1.1.5
iso-propanol
-
Bacillus thermoamylovorans
3.1.1.5
methanol
-
Bacillus thermoamylovorans
3.1.1.5
n-butanol
-
Bacillus thermoamylovorans
3.1.1.5
n-heptane
-
Bacillus thermoamylovorans
3.1.1.5
n-hexane
-
Bacillus thermoamylovorans
3.1.1.5
n-octane
-
Bacillus thermoamylovorans
3.1.1.5
n-Propanol
-
Bacillus thermoamylovorans
3.1.1.5
xylene
-
Bacillus thermoamylovorans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.5
7.7
-
4-nitrophenyl palmitate
at pH 7.5 and 55°C
Bacillus thermoamylovorans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
150000
-
gel filtration
Bacillus thermoamylovorans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.5
Bacillus thermoamylovorans
-
-
-
3.1.1.5
Bacillus thermoamylovorans BHK67
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.5
ammonium sulfate precipitation and DEAE anion exchange column chromatography
Bacillus thermoamylovorans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.5
4-nitrophenyl acetate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + acetate
-
-
-
?
3.1.1.5
4-nitrophenyl acetate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + acetate
-
-
-
?
3.1.1.5
4-nitrophenyl benzoate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + benzoate
-
-
-
?
3.1.1.5
4-nitrophenyl butyrate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + butyrate
-
-
-
?
3.1.1.5
4-nitrophenyl butyrate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + butyrate
-
-
-
?
3.1.1.5
4-nitrophenyl formate + H2O
worst substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + formate
-
-
-
?
3.1.1.5
4-nitrophenyl myristate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + myristate
-
-
-
?
3.1.1.5
4-nitrophenyl myristate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + myristate
-
-
-
?
3.1.1.5
4-nitrophenyl palmitate + H2O
best substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + palmitate
-
-
-
?
3.1.1.5
4-nitrophenyl palmitate + H2O
best substrate
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + palmitate
-
-
-
?
3.1.1.5
4-nitrophenyl stearate + H2O
second best substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + stearate
-
-
-
?
3.1.1.5
4-nitrophenyl stearate + H2O
second best substrate
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + stearate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.5
homohexamer
6 * 25000, SDS-PAGE
Bacillus thermoamylovorans
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.1.5
LPL
-
Bacillus thermoamylovorans
3.1.1.5
LYPLA1
-
Bacillus thermoamylovorans
3.1.1.5
lysophospholipase I
-
Bacillus thermoamylovorans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
55
-
the enzyme has a half-life of 10.5 h, 3.7 h and 0.5 h at 55°C, 65°C and 75°C, respectively
Bacillus thermoamylovorans
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.5
227.3
-
4-nitrophenyl palmitate
at pH 7.5 and 55°C
Bacillus thermoamylovorans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
7.5
-
-
Bacillus thermoamylovorans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
acetonitrile
-
Bacillus thermoamylovorans
3.1.1.5
DMSO
-
Bacillus thermoamylovorans
3.1.1.5
ethanol
-
Bacillus thermoamylovorans
3.1.1.5
iso-propanol
-
Bacillus thermoamylovorans
3.1.1.5
methanol
-
Bacillus thermoamylovorans
3.1.1.5
n-butanol
-
Bacillus thermoamylovorans
3.1.1.5
n-heptane
-
Bacillus thermoamylovorans
3.1.1.5
n-hexane
-
Bacillus thermoamylovorans
3.1.1.5
n-octane
-
Bacillus thermoamylovorans
3.1.1.5
n-Propanol
-
Bacillus thermoamylovorans
3.1.1.5
xylene
-
Bacillus thermoamylovorans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.5
7.7
-
4-nitrophenyl palmitate
at pH 7.5 and 55°C
Bacillus thermoamylovorans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
150000
-
gel filtration
Bacillus thermoamylovorans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
ammonium sulfate precipitation and DEAE anion exchange column chromatography
Bacillus thermoamylovorans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.5
4-nitrophenyl acetate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + acetate
-
-
-
?
3.1.1.5
4-nitrophenyl acetate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + acetate
-
-
-
?
3.1.1.5
4-nitrophenyl benzoate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + benzoate
-
-
-
?
3.1.1.5
4-nitrophenyl butyrate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + butyrate
-
-
-
?
3.1.1.5
4-nitrophenyl butyrate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + butyrate
-
-
-
?
3.1.1.5
4-nitrophenyl formate + H2O
worst substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + formate
-
-
-
?
3.1.1.5
4-nitrophenyl myristate + H2O
-
750773
Bacillus thermoamylovorans
4-nitrophenol + myristate
-
-
-
?
3.1.1.5
4-nitrophenyl myristate + H2O
-
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + myristate
-
-
-
?
3.1.1.5
4-nitrophenyl palmitate + H2O
best substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + palmitate
-
-
-
?
3.1.1.5
4-nitrophenyl palmitate + H2O
best substrate
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + palmitate
-
-
-
?
3.1.1.5
4-nitrophenyl stearate + H2O
second best substrate
750773
Bacillus thermoamylovorans
4-nitrophenol + stearate
-
-
-
?
3.1.1.5
4-nitrophenyl stearate + H2O
second best substrate
750773
Bacillus thermoamylovorans BHK67
4-nitrophenol + stearate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.5
homohexamer
6 * 25000, SDS-PAGE
Bacillus thermoamylovorans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
55
-
the enzyme has a half-life of 10.5 h, 3.7 h and 0.5 h at 55°C, 65°C and 75°C, respectively
Bacillus thermoamylovorans
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.1.5
227.3
-
4-nitrophenyl palmitate
at pH 7.5 and 55°C
Bacillus thermoamylovorans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
7.5
-
-
Bacillus thermoamylovorans