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Literature summary extracted from

  • Sharma, A.; Meena, K.; Kanwar, S.
    Molecular characterization and bioinformatics studies of a lipase from Bacillus thermoamylovorans BHK67 (2018), Int. J. Biol. Macromol., 107, 2131-2140 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 acetonitrile
-
 Caldibacillus thermoamylovorans
3.1.1.5 DMSO
-
 Caldibacillus thermoamylovorans
3.1.1.5 ethanol
-
 Caldibacillus thermoamylovorans
3.1.1.5 iso-propanol
-
 Caldibacillus thermoamylovorans
3.1.1.5 methanol
-
 Caldibacillus thermoamylovorans
3.1.1.5 n-butanol
-
 Caldibacillus thermoamylovorans
3.1.1.5 n-heptane
-
 Caldibacillus thermoamylovorans
3.1.1.5 n-hexane
-
 Caldibacillus thermoamylovorans
3.1.1.5 n-octane
-
 Caldibacillus thermoamylovorans
3.1.1.5 n-Propanol
-
 Caldibacillus thermoamylovorans
3.1.1.5 xylene
-
 Caldibacillus thermoamylovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.5 7.7
-
 4-nitrophenyl palmitate at pH 7.5 and 55°C Caldibacillus thermoamylovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 150000
-
 gel filtration Caldibacillus thermoamylovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Caldibacillus thermoamylovorans
-
-
-
3.1.1.5 Caldibacillus thermoamylovorans BHK67
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 ammonium sulfate precipitation and DEAE anion exchange column chromatography Caldibacillus thermoamylovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 4-nitrophenyl acetate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + acetate
-
 ?
3.1.1.5 4-nitrophenyl acetate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + acetate
-
 ?
3.1.1.5 4-nitrophenyl benzoate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + benzoate
-
 ?
3.1.1.5 4-nitrophenyl butyrate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + butyrate
-
 ?
3.1.1.5 4-nitrophenyl butyrate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + butyrate
-
 ?
3.1.1.5 4-nitrophenyl formate + H2O worst substrate Caldibacillus thermoamylovorans 4-nitrophenol + formate
-
 ?
3.1.1.5 4-nitrophenyl myristate + H2O
-
 Caldibacillus thermoamylovorans 4-nitrophenol + myristate
-
 ?
3.1.1.5 4-nitrophenyl myristate + H2O
-
 Caldibacillus thermoamylovorans BHK67 4-nitrophenol + myristate
-
 ?
3.1.1.5 4-nitrophenyl palmitate + H2O best substrate Caldibacillus thermoamylovorans 4-nitrophenol + palmitate
-
 ?
3.1.1.5 4-nitrophenyl palmitate + H2O best substrate Caldibacillus thermoamylovorans BHK67 4-nitrophenol + palmitate
-
 ?
3.1.1.5 4-nitrophenyl stearate + H2O second best substrate Caldibacillus thermoamylovorans 4-nitrophenol + stearate
-
 ?
3.1.1.5 4-nitrophenyl stearate + H2O second best substrate Caldibacillus thermoamylovorans BHK67 4-nitrophenol + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 homohexamer 6 * 25000, SDS-PAGE Caldibacillus thermoamylovorans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 LPL
-
 Caldibacillus thermoamylovorans
3.1.1.5 LYPLA1
-
 Caldibacillus thermoamylovorans
3.1.1.5 lysophospholipase I
-
 Caldibacillus thermoamylovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.5 55
-
 the enzyme has a half-life of 10.5 h, 3.7 h and 0.5 h at 55°C, 65°C and 75°C, respectively Caldibacillus thermoamylovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.5 227.3
-
 4-nitrophenyl palmitate at pH 7.5 and 55°C Caldibacillus thermoamylovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 7.5
-
-
 Caldibacillus thermoamylovorans