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Literature summary extracted from
- Cross-linked enzyme aggregates (CLEAs) and magnetic nanocomposite grafted CLEAs of GH26 endo-beta-1,4-mannanase Improved activity, stability and reusability (2017), Int. J. Biol. Macromol., 105, 1289-1299 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | expressed in Escherichia coli BL21 (DE3) cells | Bacillus sp. CFR1601 |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.78 | enzyme immobilization using cross-linked aggregated form (MB-C) and chitosan magnetic nanocomposites of MB-C leads to 73.2% higher activity (pH 4.0-9.0), up to 95.6% higher stability (pH 3.0-10.0, 9 h incubation at room temperature), up to 15°C higher optimal temperature, higher stability (up to 83%) in the presence of solvents and up to 1.62fold higher deactivation energy, as compared to the free enzyme. Immobilized enzymes are able to repeatedly hydrolyze locust bean gum till 12 cycles | Bacillus sp. CFR1601 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.78 | Bacillus sp. CFR1601 | A0A0A1E3J1 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | glycoprotein | - |
Bacillus sp. CFR1601 |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | phenyl Sepharose column chromatography | Bacillus sp. CFR1601 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.78 | locust bean gum + H2O | - |
Bacillus sp. CFR1601 | mannobiose + mannotriose + mannotetraose | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | endo-beta-1,4-mannanase | - |
Bacillus sp. CFR1601 |
| 3.2.1.78 | ManB-1601 | - |
Bacillus sp. CFR1601 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 70 | 85 | the free enzyme retains 42.5% residual activity after 35 min at 70-75°C. The half-lives of the free enzyme at 70, 75, 80 and 85°C are 138.6, 77, 24.7 and 25.6 min, respectively | Bacillus sp. CFR1601 |
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