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Literature summary extracted from

  • Venugopal, A.; Sudheer Kumar, C.; Siva Kumar, N.; Swamy, M.J.
    Kinetic and biophysical characterization of a lysosomal alpha-L-fucosidase from the fresh water mussel, Lamellidens corrianus (2017), Int. J. Biol. Macromol., 104, 432-441 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 L-fucose
-
 Lamellidens corrianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.85
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 5.0, 37°C Lamellidens corrianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 lysosome
-
 Lamellidens corrianus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 56000
-
 SDS-PAGE, non-denaturing PAGE Lamellidens corrianus
3.2.1.51 56175
-
 mass spectrometric analysis Lamellidens corrianus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.51 guanidine-HCl the enzyme unfolds in a broad sigmoidal, cooperative unfolding transition, centered at about 2.2 M guanidinium chloride Lamellidens corrianus
3.2.1.51 guanidinium thiocyanate the enzyme unfolds in a broad sigmoidal, cooperative unfolding transition, centered at about 2.2 M guanidinium thiocyanate Lamellidens corrianus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Lamellidens corrianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Lamellidens corrianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
 Lamellidens corrianus 4-methylumbelliferone + alpha-L-fucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 56000, SDS-PAGE Lamellidens corrianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 60
-
-
 Lamellidens corrianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.51 50 70 50°C: about 60% of maximal activity, 70°C: about 80% of maximal activity Lamellidens corrianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 1.01
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 5.0, 37°C Lamellidens corrianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5 6
-
 Lamellidens corrianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 4 7 pH 4.0: about 50% of maximal activity, pH 7.0: about 65% of maximal activity Lamellidens corrianus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 1.09
-
 L-fucose pH 5.0, 37°C Lamellidens corrianus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.51 2.5
-
 pH 5.0, 37°C Lamellidens corrianus L-fucose