EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21 Star (DE3) | Callosobruchus chinensis |
3.2.1.1 | sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21 Star (DE3) in inclusion bodies | Tribolium castaneum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | acarbose | enzyme binding and docking study | Callosobruchus chinensis | |
3.2.1.1 | acarbose | enzyme binding and docking study | Tribolium castaneum | |
3.2.1.1 | wheat amylase inhibitor | a proteinaceous inhibitor | Callosobruchus chinensis | |
3.2.1.1 | wheat amylase inhibitor | a proteinaceous inhibitor | Tribolium castaneum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
additional information | Michaelis-Menten kinetic analysis | Callosobruchus chinensis | |
3.2.1.1 | additional information | - |
additional information | Michaelis-Menten kinetic analysis | Tribolium castaneum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.1 | extracellular | the enzyme contains a signal peptide | Callosobruchus chinensis | - |
- |
3.2.1.1 | extracellular | the enzyme contains a signal peptide | Tribolium castaneum | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | starch + H2O | Callosobruchus chinensis | - |
D-glucose + maltose + maltotriose | - |
? | |
3.2.1.1 | starch + H2O | Tribolium castaneum | - |
maltose + maltotriose + maltotetraose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Callosobruchus chinensis | A0A168VCK5 | i.e. Pachymerus chinensis | - |
3.2.1.1 | Tribolium castaneum | P09107 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant His6-tagged enzyme lacking the signal peptide from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography | Callosobruchus chinensis |
3.2.1.1 | recombinant solubilized and refolded His6-tagged enzyme lacking the signal peptide from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography | Tribolium castaneum |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant His6-tagged enzyme lacking the signal peptide from Escherichia coli strain BL21 Star (DE3) inclusion bodies using solubilization of the enzyme protein in denaturing buffer containing 20 mM Tris, pH 8.5, 8 M urea, 100 mM NaCl, 1 mM 2-mercaptoethanol, followed by a on-column refolding method | Tribolium castaneum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | - |
Tribolium castaneum | ? | - |
? | |
3.2.1.1 | amylopectin + H2O | preferred substrate | Callosobruchus chinensis | ? | - |
? | |
3.2.1.1 | starch + H2O | - |
Callosobruchus chinensis | D-glucose + maltose + maltotriose | - |
? | |
3.2.1.1 | starch + H2O | - |
Tribolium castaneum | maltose + maltotriose + maltotetraose | - |
? | |
3.2.1.1 | starch + H2O | preferred substrate | Tribolium castaneum | maltose + maltotriose + maltotetraose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.1 | More | three-dimensional enzyme structure modelling | Callosobruchus chinensis |
3.2.1.1 | More | three-dimensional enzyme structure modelling | Tribolium castaneum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | CcAmy | - |
Callosobruchus chinensis |
3.2.1.1 | TcAmy | - |
Tribolium castaneum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 40 | - |
recombinant enzyme | Tribolium castaneum |
3.2.1.1 | 50 | - |
recombinant enzyme | Callosobruchus chinensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 0.92 | - |
starch | pH 5.0, 37°C, recombinant enzyme | Callosobruchus chinensis | |
3.2.1.1 | 1.1 | - |
amylopectin | pH 5.0, 37°C, recombinant enzyme | Callosobruchus chinensis | |
3.2.1.1 | 1.13 | - |
starch | pH 5.0, 37°C, recombinant enzyme | Tribolium castaneum | |
3.2.1.1 | 1.4 | - |
amylopectin | pH 5.0, 37°C, recombinant enzyme | Tribolium castaneum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 5 | - |
recombinant enzyme | Callosobruchus chinensis |
3.2.1.1 | 5 | - |
recombinant enzyme | Tribolium castaneum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.1 | evolution | phylogenetic tree of coleopteran alpha-amylases | Callosobruchus chinensis |
3.2.1.1 | evolution | phylogenetic tree of coleopteran alpha-amylases | Tribolium castaneum |
3.2.1.1 | additional information | homology modeling and molecular docking of alpha-amylase from Callosobruchus chinensis and comparison to the alpha-amylase from Tribolium castaneum showing structural differences between these two enzymes, overview | Callosobruchus chinensis |
3.2.1.1 | additional information | homology modeling and molecular docking of alpha-amylase from Callosobruchus chinensis and comparison to the alpha-amylase from Tribolium castaneum showing structural differences between these two enzymes, overview | Tribolium castaneum |