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Literature summary extracted from

  • Sulong, M.; Leow, T.; Rahman, R.; Basri, M.; Salleh, A.
    Characteristics of recombinant maltogenic amylase from Geobacillus sp. SK70 (2017), Indian J. Biotechnol., 16, 91-99 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.133 Tween-20 0.1% (v/v), stimulates Geobacillus sp. SK70

Application

EC Number Application Comment Organism
3.2.1.133 food industry potentially applied in bakery industries Geobacillus sp. SK70

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expression in Escherichia coli BL21 (DE3) Geobacillus sp. SK70

General Stability

EC Number General Stability Organism
3.2.1.133 stable in presence of 1 mM Ca2+ Geobacillus sp. SK70

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.133 2-mercaptoethanol 1% (v/v) Geobacillus sp. SK70
3.2.1.133 EDTA 1% (v/v) Geobacillus sp. SK70
3.2.1.133 SDS 1% (v/v) Geobacillus sp. SK70

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.133 Mn2+ 1 mM, stimulates Geobacillus sp. SK70
3.2.1.133 Zn2+ 1 mM, stimulates Geobacillus sp. SK70

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 135000
-
 SDS-PAGE Geobacillus sp. SK70

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Geobacillus sp. SK70
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133
-
 Geobacillus sp. SK70

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 beta-cyclodextrin + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 maltose + ?
-
 ?
3.2.1.133 pullulan + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 ?
-
 ?
3.2.1.133 starch + H2O preference of cyclodextrin as substrate over starch or pullulan Geobacillus sp. SK70 maltose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 maltogenic amylase
-
 Geobacillus sp. SK70

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 55
-
-
 Geobacillus sp. SK70

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.133 50 60 50°C: about 75% of maximal activity, 60°C: about 88% of maximal activity Geobacillus sp. SK70

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 7
-
-
 Geobacillus sp. SK70

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.133 6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 30% of maximal activity Geobacillus sp. SK70

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.133 5 10 stable Geobacillus sp. SK70