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Literature summary extracted from

  • Ravasio, V.; Damiati, E.; Zizioli, D.; Orizio, F.; Giacopuzzi, E.; Manzoni, M.; Bresciani, R.; Borsani, G.; Monti, E.
    Genomic and biochemical characterization of sialic acid acetylesterase (siae) in zebrafish (2017), Glycobiology, 27, 938-946 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.53 expressed in COS-7 cells Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.53 0.129
-
 4-nitrophenyl acetate at pH 8.7 and 25°C Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.53 endoplasmic reticulum
-
 Danio rerio 5783
-
3.1.1.53 Golgi apparatus
-
 Danio rerio 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.53 sialic acid + H2O Danio rerio
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Danio rerio Q1LUX8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.53 glycoprotein
-
 Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.53 brain
-
 Danio rerio
-
3.1.1.53 central nervous system
-
 Danio rerio
-
3.1.1.53 eye
-
 Danio rerio
-
3.1.1.53 heart
-
 Danio rerio
-
3.1.1.53 kidney
-
 Danio rerio
-
3.1.1.53 liver
-
 Danio rerio
-
3.1.1.53 muscle
-
 Danio rerio
-
3.1.1.53 ovary
-
 Danio rerio
-
3.1.1.53 pronephric duct
-
 Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 4-nitrophenyl acetate + H2O
-
 Danio rerio 4-nitrophenol + acetate
-
 ?
3.1.1.53 sialic acid + H2O
-
 Danio rerio ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 ? x * 55000, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
3.1.1.53 SIAE
-
 Danio rerio
3.1.1.53 sialic acid acetylesterase
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.53 25
-
-
 Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.53 8.7
-
-
 Danio rerio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.53 7.5 9 below pH 5.7 enzymatic activity is almost negligible. By increasing the pH from pH 7.5 to alkaline values, enzyme activity reaches its maximum value at pH 8.7, followed by a drastic decrease at pH 9.0 with a residual activity of 32.8% Danio rerio

Expression

EC Number Organism Comment Expression
3.1.1.53 Danio rerio the enzyme transcript is always detectable during development, with a more specific expression in the central nervous system, in pronephric ducts and liver in the more advanced stages of the embryo development up