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Literature summary extracted from

  • Silchenko, A.S.; Ustyuzhanina, N.E.; Kusaykin, M.I.; Krylov, V.B.; Shashkov, A.S.; Dmitrenok, A.S.; Usoltseva, R.V.; Zueva, A.O.; Nifantiev, N.E.; Zvyagintseva, T.N.
    Expression and biochemical characterization and substrate specificity of the fucoidanase from Formosa algae (2017), Glycobiology, 27, 254-263 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.212 Ba2+ activates Formosa algae
3.2.1.212 Ca2+ activates Formosa algae
3.2.1.212 Co2+ activates Formosa algae
3.2.1.212 Mg2+ activates Formosa algae
3.2.1.212 Mn2+ activates Formosa algae

Application

EC Number Application Comment Organism
3.2.1.212 synthesis the enzyme can be used for the modification of natural fucoidans to obtain more regular and easier characterized derivatives useful for research and practical applications Formosa algae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.212 expression in Escherichia coli Formosa algae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.212 Al3+
-
 Formosa algae
3.2.1.212 Cu2+
-
 Formosa algae
3.2.1.212 EDTA the addition of EDTA accompanied by desalting of a medium completely inactivates the enzyme Formosa algae
3.2.1.212 Fe3+
-
 Formosa algae
3.2.1.212 Sn2+
-
 Formosa algae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.212 101200
-
 calculated from sequence Formosa algae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.212 Formosa algae WP_057784219.1
-
-
3.2.1.212 Formosa algae KMM 3553T WP_057784219.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.212
-
 Formosa algae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.212 fucoidan + H2O fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively Formosa algae sulfated alpha-L-fucooligosaccharides
-
 ?
3.2.1.212 fucoidan + H2O fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively Formosa algae KMM 3553T sulfated alpha-L-fucooligosaccharides
-
 ?
3.2.1.212 additional information unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) Formosa algae ?
-
 ?
3.2.1.212 additional information unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) Formosa algae KMM 3553T ?
-
 ?
3.2.1.212 propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O
-
 Formosa algae propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)
-
 ?
3.2.1.212 propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O
-
 Formosa algae KMM 3553T propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)
-
 ?
3.2.1.212 propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O efficiently cleaved to form a complex mixture of mono- and oligosaccharides Formosa algae ?
-
 ?
3.2.1.212 propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O efficiently cleaved to form a complex mixture of mono- and oligosaccharides Formosa algae KMM 3553T ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.212 FFA2
-
 Formosa algae
3.2.1.212 poly[(1->4)-alpha-L-fucoside-2-sulfate] glycanohydrolase
-
 Formosa algae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.212 25 37
-
 Formosa algae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.212 6.5 9
-
 Formosa algae