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Literature summary extracted from

  • Liu, S.; Kulinich, A.; Cai, Z.P.; Ma, H.Y.; Du, Y.M.; Lv, Y.M.; Liu, L.; Voglmeir, J.
    The fucosidase-pool of Emticicia oligotrophica Biochemical characterization and transfucosylation potential (2016), Glycobiology, 26, 871-879 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.51 analysis the facile recombinant expression procedure and the peculiar substrate specificities make the enzyme an attractive candidates for bioanalytical applications Emticicia oligotrophica
3.2.1.51 analysis the facile recombinant expression procedure and the peculiar substrate specificities make these enzymes attractive candidates for bioanalytical applications Emticicia oligotrophica
3.2.1.51 analysis tool for the analysis and modification of human and animal milk oligosaccharides Emticicia oligotrophica
3.2.1.51 synthesis the transfucosylation ability of one of the fucosidase isoform might be of interest for the synthesis of fucosides Emticicia oligotrophica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expression in Escherichia coli BL21 Emticicia oligotrophica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 Ca2+ 1 mM, 47% loss of activity of Eo3066 Emticicia oligotrophica
3.2.1.51 Mn2+ 1 mM, 39% loss of activity of Eo3066 Emticicia oligotrophica
3.2.1.51 Zn2+ 1 mM, 42% loss of activity of Eo3066 Emticicia oligotrophica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.75
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 8.41
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 8.63
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Ca2+ 1 mM, 50% increase in activity Emticicia oligotrophica
3.2.1.51 Ca2+ 1 mM, 133% increase in activity Emticicia oligotrophica
3.2.1.51 Mg2+ 1 mM, 76% increase in activity Emticicia oligotrophica
3.2.1.51 Mn2+ 1 mM, 56% increase in activity Emticicia oligotrophica
3.2.1.51 Mn2+ 1 mM, 64% increase in activity Emticicia oligotrophica
3.2.1.51 Zn2+ 1 mM, 99% increase in activity Emticicia oligotrophica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 52000
-
 SDS-PAGE Emticicia oligotrophica
3.2.1.51 55000
-
 SDS-PAGE Emticicia oligotrophica
3.2.1.51 59000
-
 SDS-PAGE Emticicia oligotrophica
3.2.1.51 100000
-
 more than 100000 Da, non-denaturing PAGE Emticicia oligotrophica

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Emticicia oligotrophica
-
-
-
3.2.1.51 Emticicia oligotrophica DSM 17448
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Emticicia oligotrophica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 0.3
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 0.34
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 2.22
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 6.5, 37°C Emticicia oligotrophica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 3-fucosyllactose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica L-fucose +
-
 ?
3.2.1.51 3-fucosyllactose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica DSM 17448 L-fucose +
-
 ?
3.2.1.51 3-fucosyllactose + H2O cleavage of alpha-1,3 bond Emticicia oligotrophica L-fucose + ?
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Emticicia oligotrophica 4-nitrophenol + L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Emticicia oligotrophica DSM 17448 4-nitrophenol + L-fucose
-
 ?
3.2.1.51 lacto-N-difucohexaose I + H2O cleavage of alpha-1,4 bond Emticicia oligotrophica L-fucose + ?
-
 ?
3.2.1.51 lacto-N-difucohexaose II + H2O cleavage of alpha-1,4 bond Emticicia oligotrophica L-fucose + ?
-
 ?
3.2.1.51 lacto-N-fucopentaose + H2O cleavage of alpha-1,3 bond Emticicia oligotrophica L-fucose +
-
 ?
3.2.1.51 lacto-N-fucopentaose + H2O cleavage of alpha-1,3 bond, low efficiency Emticicia oligotrophica L-fucose +
-
 ?
3.2.1.51 mono-sialylated-galactosylated biantennary N-glycan-core-substituted with alpha-(1->6)-linked fucose + H2O
-
 Emticicia oligotrophica L-fucose + ?
-
 ?
3.2.1.51 additional information the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency Emticicia oligotrophica ?
-
 ?
3.2.1.51 additional information the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively Emticicia oligotrophica ?
-
 ?
3.2.1.51 additional information the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides Emticicia oligotrophica ?
-
 ?
3.2.1.51 additional information the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency Emticicia oligotrophica DSM 17448 ?
-
 ?
3.2.1.51 additional information the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively Emticicia oligotrophica DSM 17448 ?
-
 ?
3.2.1.51 additional information the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides Emticicia oligotrophica DSM 17448 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 ? x * 55000, SDS-PAGE Emticicia oligotrophica
3.2.1.51 homooligomer x * 52000, SDS-PAGE Emticicia oligotrophica
3.2.1.51 homooligomer x * 59000, SDS-PAGE Emticicia oligotrophica

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 Eo0918
-
 Emticicia oligotrophica
3.2.1.51 Eo3066
-
 Emticicia oligotrophica
3.2.1.51 Eo3812
-
 Emticicia oligotrophica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 30
-
-
 Emticicia oligotrophica
3.2.1.51 37
-
 assay at Emticicia oligotrophica
3.2.1.51 42
-
-
 Emticicia oligotrophica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.51 15 42 5°C: about 55% of maximal activity, 42°C: about 50% of maximal activity Emticicia oligotrophica
3.2.1.51 25 50 25°C: about 70% of maximal activity, 50°C: about 30% of maximal activity Emticicia oligotrophica
3.2.1.51 30 50 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity Emticicia oligotrophica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 42
-
 activity decreases rapidly above 42°C Emticicia oligotrophica
3.2.1.51 60
-
 almost complete inactivation Emticicia oligotrophica
3.2.1.51 70
-
 almost complete inactivation Emticicia oligotrophica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 0.04
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 3.61
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica
3.2.1.51 3.73
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.5, 37°C Emticicia oligotrophica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 6
-
-
 Emticicia oligotrophica
3.2.1.51 6.5
-
-
 Emticicia oligotrophica
3.2.1.51 6.5
-
 assay at Emticicia oligotrophica
3.2.1.51 7
-
-
 Emticicia oligotrophica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 5.5 7 pH 5.5: about 40% of maximal activity, pH 7.0: about 60% of maximal activity Emticicia oligotrophica
3.2.1.51 5.5 7 pH 5.5: about 60% of maximal activity, pH 7.0: about 70% of maximal activity Emticicia oligotrophica
3.2.1.51 6 8.5 pH 6.0: about 30% of maximal activity, pH 8.5: about 50% of maximal activity Emticicia oligotrophica