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Literature summary extracted from

  • Orizio, F.; Damiati, E.; Giacopuzzi, E.; Benaglia, G.; Pianta, S.; Schauer, R.; Schwartz-Albiez, R.; Borsani, G.; Bresciani, R.; Monti, E.
    Human sialic acid acetyl esterase Towards a better understanding of a puzzling enzyme (2015), Glycobiology, 25, 992-1006 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.53 expressed in COS-7 cells Homo sapiens
3.1.1.53 expression in COS7 cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.53 H377A complete loss of activity Homo sapiens
3.1.1.53 H377A the mutation abolishes the enzymatic activity Homo sapiens
3.1.1.53 S332A no significant influence on activity Homo sapiens
3.1.1.53 S332A the mutation does not influence the activity of the enzyme significantly Homo sapiens
3.1.1.53 T132A complete loss of activity Homo sapiens
3.1.1.53 T132A the mutation abolishes the enzymatic activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.53 endoplasmic reticulum
-
 Homo sapiens 5783
-
3.1.1.53 extracellular
-
 Homo sapiens
-
-
3.1.1.53 Golgi apparatus
-
 Homo sapiens 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.53 sialic acid + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Homo sapiens
-
-
-
3.1.1.53 Homo sapiens Q9HAT2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.53 glycoprotein glycosylation influences the biological activity of the enzyme and is essential for release of the enzyme into the culture medium Homo sapiens
3.1.1.53 glycoprotein N-glycosylation sites: N107, N138, N267, N290, N401, N422. O-glycosylation sites: S177, S231, S242, T511 Homo sapiens
3.1.1.53 phosphoprotein phosphorylation sites: S30, Y31, T52, S74, S79, S144, S156, S158, S217, T221, S227, S228, S247, S255, T303, S308, T312, S328, S331, S332, Y349, S371, T461, S470 Homo sapiens
3.1.1.53 proteolytic modification the 58000 kDa form of SIAE is rapidly proteolitically cleaved to a 49000 Da form or glycosylated to the 62000 Da form Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.53 colon high expression level Homo sapiens
-
3.1.1.53 kidney high expression level Homo sapiens
-
3.1.1.53 additional information gene is expressed in all tissues tested, although at different levels Homo sapiens
-
3.1.1.53 thyroid gland high expression level Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
3.1.1.53 sialic acid + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 ? x * 62000, SDS-PAGE Homo sapiens
3.1.1.53 ? x * 58000, calculated from sequence, x * 62000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.53 SIAE
-
 Homo sapiens
3.1.1.53 sialic acid acetyl esterase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.53 8.4 8.5
-
 Homo sapiens
3.1.1.53 8.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.53 6
-
 negligible activity below Homo sapiens