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Literature summary extracted from

  • Schneble, N.; Mueller, J.; Kliche, S.; Bauer, R.; Wetzker, R.; Boehmer, F.D.; Wang, Z.Q.; Mueller, J.P.
    The protein-tyrosine phosphatase DEP-1 promotes migration and phagocytic activity of microglial cells in part through negative regulation of fyn tyrosine kinase (2017), Glia, 65, 416-428 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 gene Ptprj, quantitative PCR expression analysis Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.48 additional information generation of specific shRNA cell lines for DEP-1 knockout Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.48 Mn2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 additional information Mus musculus in vivo phagocytosis assay and in vivo microglial migration assay, overview ?
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 ?
3.1.3.48 additional information Mus musculus C57BL/6 in vivo phagocytosis assay and in vivo microglial migration assay, overview ?
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mus musculus
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mus musculus C57BL/6
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Mus musculus Q64455
-
-
3.1.3.48 Mus musculus C57BL/6 Q64455
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.48 brain
-
 Mus musculus
-
3.1.3.48 BV-2 cell
-
 Mus musculus
-
3.1.3.48 cerebral cortex
-
 Mus musculus
-
3.1.3.48 microglia murine microglia are isolated from newborn mice, DEP-1 is expressed in microglial cells in a cell density-dependent manner Mus musculus
-
3.1.3.48 additional information DEP-1 protein levels increase in several cell types at high-cell density. DEP-1 is a widely expressed in cells of the immune system Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 additional information in vivo phagocytosis assay and in vivo microglial migration assay, overview Mus musculus ?
-
 ?
3.1.3.48 additional information in vivo phagocytosis assay and in vivo microglial migration assay, overview Mus musculus C57BL/6 ?
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mus musculus [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mus musculus C57BL/6 [a protein]-tyrosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 CD148
-
 Mus musculus
3.1.3.48 density-enhanced phosphatase-1
-
 Mus musculus
3.1.3.48 DEP-1
-
 Mus musculus
3.1.3.48 DEP-1 phosphatase
-
 Mus musculus
3.1.3.48 protein-tyrosine phosphatase
-
 Mus musculus
3.1.3.48 PTP
-
 Mus musculus
3.1.3.48 PTPRJ
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 7.5
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
3.1.3.48 malfunction in the microglial cell line BV-2, DEP-1 depletion by shRNA-mediated knockdown results in enhanced phosphorylation of the Fyn activating tyrosine (Tyr420) and elevated specific Fyn-kinase activity in immunoprecipitates. Fyn mRNA and protein levels are reduced in DEP-1-deficient microglia cells. Knockdown of DEP-1 causes a small but significant decrease of phagocytosis of unstimulated microglial BV-2 cells. And the stimulatory effect on phagocytosis is significantly reduced in cells depleted for DEP-1, reduced phagocytic activity of microglial cells in DEP-1-/- mice brains Mus musculus
3.1.3.48 physiological function density-enhanced phosphatase-1 (DEP-1), also designated PTPRJ, receptor-type PTP eta or CD148, is a transmembrane PTP endowed with one intracellular catalytic domain, and an extracellular domain harboring eight fibronectin-like repeats. The protein-tyrosine phosphatase DEP-1 promotes migration and phagocytic activity of microglial cells in part through negative regulation of fyn tyrosine kinase. DEP-1 protein levels increase in several cell types at high-cell density, suggesting a role of the molecule in density-dependent growth control. A negative regulatory role of Fyn for microglial functions is inhibited by DEP-1. Determination of a role of DEP-1 in a potential DEP-1-Fyn axis in the regulation of microglial functions. Enzyme DEP-1 stimulates microglial phagocytosis and microglial cell migration, overview Mus musculus