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Literature summary extracted from

  • Gil-Duran, C.; Ravanal, M.C.; Ubilla, P.; Vaca, I.; Chavez, R.
    Heterologous expression, purification and characterization of a highly thermolabile endoxylanase from the Antarctic fungus Cladosporium sp (2018), Fungal Biol., 122, 875-882 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Pichia pastoris Cladosporium sp.
3.2.1.8 gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His- and c-myc-tagged enzyme in Pichia pastoris strain GS115, the enzyme is secreted Cladosporium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 34400
-
 calculated from sequence Cladosporium sp.
3.2.1.8 37000
-
 SDS-PAGE Cladosporium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Cladosporium sp. A0A346PXS9
-
-
3.2.1.8 Cladosporium sp. A0A346PXS9 isolated from Antarctica
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 additional information no N- or O-glycosylations sites are detected by bioinformatics tools Cladosporium sp.
3.2.1.8 no glycoprotein no N- or O-glycosylations sites are detected by bioinformatic tools Cladosporium sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Cladosporium sp.
3.2.1.8 recombinant extracellular His- and c-myc-tagged enzyme from Pichia pastoris strain GS115 by anion exchange and cation exchange chromatography Cladosporium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.8 mycelium Cladosporium sp. is grown on CM medium at 15°C and 180 rpm during 72 h Cladosporium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 728
-
 purified extracellular recombinant enzyme, pH 6.0, 50°C, substrate rye arabinoxylan Cladosporium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 arabinoxylan + H2O the enzyme is most active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan). It releases xylooligosaccharides but not xylose, indicating that XynA is an endoxylanase Cladosporium sp. xylobiose + xylotriose + xylotetraose + ?
-
 ?
3.2.1.8 beechwood xylan + H2O 62.6% activity compared to rye arabinoxylan Cladosporium sp. ?
-
 ?
3.2.1.8 birchwood xylan + H2O 32.4% activity compared to rye arabinoxylan Cladosporium sp. ?
-
 ?
3.2.1.8 additional information the enzyme is more active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan) Cladosporium sp. ?
-
 ?
3.2.1.8 oat spelt xylan + H2O 61.9% activity compared to rye arabinoxylan Cladosporium sp. ?
-
 ?
3.2.1.8 rye arabinoxylan + H2O best substrate Cladosporium sp. ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O 92.0% activity compared to rye arabinoxylan Cladosporium sp. ?
-
 ?
3.2.1.8 xylan + H2O the enzyme is most active on xylans with high content of arabinose (rye arabinoxylan and wheat arabinoxylan) than on xylans with low content of arabinose (oat spelts xylan, birchwood xylan and beechwood xylan). It releases xylooligosaccharides but not xylose, indicating that XynA is an endoxylanase Cladosporium sp. xylobiose + xylotriose + xylotetraose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 34441, sequence calculation, x * 37000, recombinant extracellular His- and c-myc-tagged enzyme, SDS-PAGE Cladosporium sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 beta-xylanase
-
 Cladosporium sp.
3.2.1.8 endoxylanase
-
 Cladosporium sp.
3.2.1.8 XynA
-
 Cladosporium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
-
 Cladosporium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 20 60 20°C: about 40% of maximal activity, 60°C: about 35% of maximal activity Cladosporium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 5 25 purified recombinant enzyme, 3 h, over 90% activity remaining Cladosporium sp.
3.2.1.8 30
-
 3 h, 50% loss of activity Cladosporium sp.
3.2.1.8 30
-
 purified recombinant enzyme, 3 h, loss of 50% activity Cladosporium sp.
3.2.1.8 35
-
 1 h, 50% loss of activity Cladosporium sp.
3.2.1.8 35
-
 purified recombinant enzyme, 1 h, loss of 50% activity Cladosporium sp.
3.2.1.8 40
-
 20-30 min, complete inactivation Cladosporium sp.
3.2.1.8 40
-
 purified recombinant enzyme, 20-30 min, complete inactivation Cladosporium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6 7
-
 Cladosporium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 35% of maximal activity Cladosporium sp.

General Information

EC Number General Information Comment Organism
3.2.1.8 evolution the enzyme belongs to the glycoside hydrolase family 10, GH10 Cladosporium sp.