Any feedback?
Literature summary extracted from
- The GbsR family of transcriptional regulators functional characterization of the OpuAR repressor (2018), Front. Microbiol., 9, 2536 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.9 | gene opuAB, sequence comparisons | Bacillus subtilis |
| 7.6.2.9 | gene opuAC, sequence comparisons | Bacillus subtilis |
| 7.6.2.9 | opuA operon, the coding region of the opuA operon and the adjacent opuAR regulatory gene are cloned into the vector pX resulting in plasmid pCA-opuARA, sequence comparisons, recombinant expression in Bacillus subtilis strain TMB118, in which only the proline-specific osmostress OpuE transporter is present, the resulting strain is CAB2. opuAA0 Bacillus infantis-treA reporter gene fusions are constructed using primers opuAR treA Frag1/2 rev and either opuAR treA Frag1/4. Recombinant overexpression of StrepII-tagged OpuAR repressor and mutant derivatives from the P-tet promoter in the Escherichia coli B strain BL21 | Bacillus infantis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 7.6.2.9 | OpuBC protein crystal structure analysis, PDB ID 3R6U | Bacillus subtilis |
| 7.6.2.9 | OpuBC protein crystal structure analysis, PDB ID 3R6U | Bacillus infantis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.9 | additional information | - |
additional information | dissociation constants of recombinant OpuAR for choline and glycine betaine are determined by intrinsic tryptophan fluorescence spectroscopy | Bacillus infantis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.9 | Mg2+ | required | Bacillus subtilis |  |
| 7.6.2.9 | Mg2+ | required | Bacillus infantis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | Bacillus subtilis | - |
ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | Bacillus infantis | - |
ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | Bacillus subtilis 168 | - |
ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | Bacillus infantis NRRL B-14911 | - |
ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.9 | Bacillus infantis | U5LFB4 AND U5LHK3 AND U5LHN4 | opuA operon, genes opuAA, opuAB, and opuAC encoding glycine betaine transport ATP-binding protein OpuAA (quaternary-amine-transporting ATPase), glycine betaine transport system permease protein OpuAB, and glycine betaine-binding protein OpuAC | - |
| 7.6.2.9 | Bacillus infantis NRRL B-14911 | U5LFB4 AND U5LHK3 AND U5LHN4 | opuA operon, genes opuAA, opuAB, and opuAC encoding glycine betaine transport ATP-binding protein OpuAA (quaternary-amine-transporting ATPase), glycine betaine transport system permease protein OpuAB, and glycine betaine-binding protein OpuAC | - |
| 7.6.2.9 | Bacillus subtilis | P46921 | gene opuAB encoding glycine betaine transport system permease protein OpuAB | - |
| 7.6.2.9 | Bacillus subtilis | P46922 | gene opuAC glycine betaine-binding protein OpuAC | - |
| 7.6.2.9 | Bacillus subtilis 168 | P46921 | gene opuAB encoding glycine betaine transport system permease protein OpuAB | - |
| 7.6.2.9 | Bacillus subtilis 168 | P46922 | gene opuAC glycine betaine-binding protein OpuAC | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.9 | recombinant wild-type and mutant OpuAR repressor proteins from Escherichia coli strain BL21 by affinity chromatography and gel filtration, determination of quaternary assembly of purified OpuAR protein | Bacillus infantis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | - |
Bacillus subtilis | ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | - |
Bacillus infantis | ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | - |
Bacillus subtilis 168 | ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
| 7.6.2.9 | ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] | - |
Bacillus infantis NRRL B-14911 | ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.9 | glycine betaine transport system permease protein | UniProt | Bacillus subtilis |
| 7.6.2.9 | glycine betaine-binding protein OpuAC | UniProt | Bacillus subtilis |
| 7.6.2.9 | glycine/betaine ABC transporter | UniProt | Bacillus infantis |
| 7.6.2.9 | N288_21505 | - |
Bacillus infantis |
| 7.6.2.9 | N288_21525 | - |
Bacillus infantis |
| 7.6.2.9 | N288_25665 | - |
Bacillus infantis |
| 7.6.2.9 | OpuAB | - |
Bacillus subtilis |
| 7.6.2.9 | OpuB | - |
Bacillus subtilis |
| 7.6.2.9 | OpuC | - |
Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.9 | ATP | - |
Bacillus subtilis | |
| 7.6.2.9 | ATP | - |
Bacillus infantis | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 7.6.2.9 | Bacillus subtilis | the MarR-type regulator GbsR, which acts as an intracellular choline-responsive repressor, controls the transcription of the opuB and gbsAB operons | down |
| 7.6.2.9 | Bacillus infantis | the MarR-type regulator GbsR, which acts as an intracellular choline-responsive repressor, controls the transcription of the opuB and gbsAB operons | down |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.6.2.9 | evolution | the amino acid sequences of the components of Bacillus subtilis OpuB and OpuC ABC transporters are closely related to each other because the opuB and opuC operon are likely the result of a gene duplication event. The least conserved component of these two transporter systems are their substrate binding proteins (OpuBC and OpuCC, respectively) with a degree of amino acid sequence identity of 71% of the mature proteins. Gene opuAA (UniProt ID O32243) encodes the glycine betaine transport ATP-binding protein OpuAA (quaternary-amine-transporting ATPase) | Bacillus subtilis |
| 7.6.2.9 | evolution | the amino acid sequences of the components of Bacillus subtilis OpuB and OpuC ABC transporters are closely related to each other because the opuB and opuC operon are likely the result of a gene duplication event. The least conserved component of these two transporter systems are their substrate binding proteins (OpuBC and OpuCC, respectively) with a degree of amino acid sequence identity of 71% of the mature proteins. Gene opuAA (UniProt ID O32243) encodes the glycine betaine transport ATP-binding protein OpuAA (quaternary-amine-transporting ATPase). OpuA-type ABC transporters are identified by assessing the amino acid sequence relatedness with the OpuAC substrate-binding protein from Bacillus subtilis | Bacillus subtilis |
| 7.6.2.9 | evolution | the genome sequence of Bacillus infantis NRRL B-14911 predicts in addition to OpuA, the presence of several other types of osmostress protectant uptake systems (e.g. OpuF, OpuD, OpuE) | Bacillus infantis |
| 7.6.2.9 | physiological function | five transporters for osmostress protectants (Opu) have been characterized in the systems for production of the compatible solutes proline and glycine betaine. Glycine betaine synthesis relies on the import of choline via the substrate-restricted OpuB system and the promiscuous OpuC transporter and its subsequent oxidation by the GbsAB enzymes. Transcription of the opuB and gbsAB operons is under control of the MarR-type regulator GbsR, which acts as an intracellular choline-responsive repressor. GbsR-/OpuAR-type proteins are an extended subgroup within the MarR-superfamily of transcriptional regulators and an additional type of substrate-inducible import system for osmostress protectants. The presence of either glycine betaine or proline betaine affords a substantial level of osmostress protection. Choline serves as the inducer for relief of GbsR-mediated repression of the Bacillus subtilis gbsAB and opuB operons | Bacillus subtilis |
| 7.6.2.9 | physiological function | five transporters for osmostress protectants (Opu) have been characterized in the systems for production of the compatible solutes proline and glycine betaine. Glycine betaine synthesis relies on the import of choline via the substrate-restricted OpuB system and the promiscuous OpuC transporter and its subsequent oxidation by the GbsAB enzymes. Transcription of the opuB and gbsAB operons is under control of the MarR-type regulator GbsR, which acts as an intracellular choline-responsive repressor. GbsR-/OpuAR-type proteins are an extended subgroup within the MarR-superfamily of transcriptional regulators and an additional type of substrate-inducible import system for osmostress protectants. The presence of either glycine betaine or proline betaine affords a substantial level of osmostress protection. Since the genome sequence of Bacillus infantis NRRL B-14911 lacks glycine betaine synthesis genes, it is readily understandable why choline is not osmostress protective, as this compound is not a compatible solute per se because its osmostress-relieving properties depend on its enzymatic conversion into glycine betaine | Bacillus subtilis |
| 7.6.2.9 | physiological function | five transporters for osmostress protectants (Opu) have been characterized in the systems for production of the compatible solutes proline and glycine betaine. Glycine betaine synthesis relies on the import of choline via the substrate-restricted OpuB system and the promiscuous OpuC transporter and its subsequent oxidation by the GbsAB enzymes. Transcriptional regulation of the Bacillus infantis opuA gene cluster via the GbsR-type regulator OpuAR, the Bacillus infantis GbsR protein is the OpuAR regulatory protein. GbsR-/OpuAR-type proteins are an extended subgroup within the MarR-superfamily of transcriptional regulators and an additional type of substrate-inducible import system for osmostress protectants. The presence of either glycine betaine or proline betaine affords a substantial level of osmostress protection. The major substrates (glycine betaine and proline betaine) of Bacillus infantis OpuA ABC importer also serve as inducers of OpuAR to relieve the DNA-binding activity of this repressor protein | Bacillus infantis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
