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Literature summary extracted from

  • Li, H.; Wu, M.; Shi, Y.; Javid, B.
    Over-expression of the mycobacterial trehalose-phosphate phosphatase OtsB2 results in a defect in macrophage phagocytosis associated with increased mycobacterial-macrophage adhesion (2016), Front. Microbiol., 7, 1754 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.12 gene OTB2, overexpression in Mycobacterium bovis strain BCG / Pasteur 1173P2, a Mycobacterium tuberculosis variant bovis BCG, from plasmid pMV261, subcloning in Escherichia coli strain DH5alpha Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.12 additional information effects of OtsB2 expression in Mycobacterium tuberculosis variant bovis BCG on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-bacillus calmette-guerin (BCG) overexpressing enzyme OtsB2 is able to better survive in stationary phase. Overexpression of enzyme OtsB2 leads to a decrease in phagocytosis but not survival in human monocyte cell-line THP-1, macrophage-like cells, and this is not due to a decrease in general macrophage phagocytic activity. Strain BCG overexpressing enzyme OtsB2 have stronger binding to THP-1 cells than wild-type BCG. Altering OtsB2 expression has implications for mycobacterial host-pathogen interactions, overview Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.12 alpha,alpha-trehalose 6-phosphate + H2O Mycobacterium tuberculosis
-
 alpha,alpha-trehalose + phosphate
-
 ?
3.1.3.12 alpha,alpha-trehalose 6-phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 alpha,alpha-trehalose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Mycobacterium tuberculosis P9WFZ5
-
-
3.1.3.12 Mycobacterium tuberculosis H37Rv P9WFZ5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 alpha,alpha-trehalose 6-phosphate + H2O
-
 Mycobacterium tuberculosis alpha,alpha-trehalose + phosphate
-
 ?
3.1.3.12 alpha,alpha-trehalose 6-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv alpha,alpha-trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.12 otsB2
-
 Mycobacterium tuberculosis
3.1.3.12 TPP
-
 Mycobacterium tuberculosis
3.1.3.12 trehalose-6-phosphate phosphatase
-
 Mycobacterium tuberculosis
3.1.3.12 trehalose-phosphate phosphatase
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.3.12 physiological function trehalose-6-phosphate phosphatase (OtsB2) is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth Mycobacterium tuberculosis