EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.20 | additional information | enzyme immobilization onto an epoxy-activated resin (Eupergit C), improved synthesis of isomaltooligosaccharides (IMOs) using immobilized alpha-glucosidase in organic-aqueous media, method optimization, overview. An immobilization efficiency of 79.61% is obtained under the condition of pH 6.0, ionic strength of 2.0 M, and 30 mg of protein/g of resin. The butyl acetate-aqueous biphasic system significantly improves the catalytic activity of the immobilized enzyme and the yield of IMOs. The highest yield of IMOs (50.83% w/w) is obtained at pH 4.5 and 55°C in a butyl acetate/buffer system (25:75 v/v) | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | additional information | - |
additional information | kinetics of transglucosylation activity of immobilized alpha-glucosidase | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Aspergillus niger | A0PCH8 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.20 | commercial preparation | - |
Aspergillus niger | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | additional information | immobilized recombinant alpha-glucosidase catalyzes maltose into isomaltooligosaccharides (IMOs) by its transglucosylation activity. The synthesis of IMOs is conducted using maltose as the substrate | Aspergillus niger | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | AGL | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 55 | - |
- |
Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 5 | - |
- |
Aspergillus niger |